During the 2010 monsoon season, Phytophthora insolita was isolated from water accumulated under the canopy of a Nagpur mandarin tree (Citrus reticulata) in Nagpur, Maharashtra State, India. The identity of the isolate was confirmed by morphological and molecular methods. This is the first report of P. insolita from India.
The widespread occurrence of Huanglongbing (HLB) was recorded in sixteen citrus growing states of India using the real-time quantitative PCR and the derived threshold cycle (Ct) value. All the commercially important citrus varieties of mandarin, sweet orange, lime and lemon, pummelo and Satkara were infected with 'Candidatus Liberibacter asiaticus', the bacterium associated with HLB. Ct values positive for HLB were found in all the states except Arunachal Pradesh. The primer-probe combination HLBasHLBr-HLBp was found specific to Ca. L. asiaticus and do not exhibit any cross-reactivity with other pathogenic residents of citrus.
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