Grafting with poly(N-vinyl-2-pyrrolidone) (PVP)
was used to functionalize silica. The functionalized silicas were
characterized by scanning electron microscopy, nitrogen adsorption,
energy-dispersive X-ray analysis, Fourier transform spectroscopy,
and thermogravimetric analysis. The Barrett–Joyner–Halenda
analysis confirmed that PVP-modified silica samples have a mesoporous
structure. Bovine serum albumin (BSA) was immobilized onto the surface
of PVP-modified silica systems through physical adsorption. Also it
was shown that the adsorption capacity of the tested samples depends
on the quantity of PVP inside the silica matrix, which confirmed the
formation of a specific complex between BSA and PVP. In addition,
the effects of pH, time, and concentration on BSA adsorption were
investigated. The maximum BSA adsorption capacity was 71.54 ±
2.0 mg/g. The desorption and reusability of all prepared samples were
studied as well. PVP-modified silicas are capable of holding more
BSA compared to nonmodified silica. These characteristics indicate
that PVP-modified silicas have great potential for efficient protein
encapsulation.
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