India being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be a development of rapid, reliable, robust diagnostic technique, which can work with high degree of sensitivity and specificity. Loop mediated isothermal amplification assay is a rapid gene amplification technique that amplifies nucleic acid under an isothermal condition with a set of designed primers spanning eight distinct sequences of the target. This assay can be used as an emerging powerful, innovative gene amplification diagnostic tool against various pathogens of livestock diseases. This review is to highlight the basic concept and methodology of this assay in livestock disease.

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The world has been plagued by an unforeseen threat to public health by the appearance of COVID-19, which has impacted the world’s economy and the worldwide supply chain. The unexpected pandemic (COVID-19) quickly spread across the world, leaving not a single country unaffected by the worldwide pandemic. At the start of COVID-19, several countries adopted social distancing and lockdowns due to the virus. The existing pandemic’s discriminatory aspect has a negative influence on human health and the standard of living, as well as worldwide trade, supply chains, and major economies. COVID-19 has been shown to have an effect throughout the broader food supply chain, one of the largest significant sectors of any country, from the field to the consumers. There are currently substantial concerns regarding food production, manufacturing, delivery, and consumption in light of emerging issues within the food supply chain. Limitations on the mobility of workers, variations in consumer demands, the shutdown of food manufacturing industries, limited food trading regulations, and financial stress throughout the food supply chain are all results of the COVID-19 pandemic. The pandemic (COVID-19) has changed consumer behavior and affected the business and economic sector. The purpose of this study was to explore the application of the Q-technique in the investigation of online consumer and manufacturer behavior in relation to end-user food purchases via digital marketing skills, supply chain possibilities, food purchaser and consumer happiness, and e-commerce infrastructure efficiency under the influence of COVID-19. The results show that in the crisis of supply chain management, clarifying the import food industry e-commerce supply chain crisis items, and determining their priority and strength can help enterprises make emergency decisions regarding supply chain operation; they can also be used as a reference for enterprises to use in responding to the crisis.

Aim:A study on age-related electrocardiographic (ECG) changes was conducted on 20 apparently healthy Black Bengal goats with no history of cardiac disorders during 2015-2016.Materials and Methods:The goats selected for the study belonged to four different age groups; Group 1: Goats up to 6 months of age, Group 2: Above 6 months and below 1 year of age, Group 3: Above 1 year and below 2 years of age, and Group 4: Above 2 years of age. The ECG was recorded with the animals in standing position using a 12-lead standard ECG recorder (Model-Cardiart-108 MK VII, manufactured by BPL, India). The paper speed was set to 25 mm/s with the sensitivity of the machine was adjusted at 1 (1 cm=mV).Results:The ECG parameters were compared within different age groups, and the data were analyzed statistically using SPSS 16.0 taking a significant level of 95% (p<0.05) in all cases. The lead-I ECG revealed a significant difference in amplitude of QRS complex, PR interval, QT interval, RR interval, PQ segment, ST segment, TP segment, and heart rate among some age groups. In bipolar limb lead-II, the amplitude of T-wave, RR interval, ST segment, TP segment, and heart rate was a significant difference among some age groups. Lead-III presented significant difference among age groups in different parameters such as QRS complex duration, T-wave duration, RR interval, ST segment, TP segment, and heart rate.Conclusion:The study concluded that there is a significant variation in the ECG parameters both in terms of values and configuration of ECG waves when age is taken into consideration. The results of the study might be used as a reference value for field veterinarians.

A field experiment was conducted in the Regional Research Technology and Transfer Station (RRTTS), Chiplima, Sambalpur for two years to study the effect of different sources and levels on growth, yield and uptake of sulphur in onion (cv. Nasik Red). The treatment consists of two sources of sulphur (elemental gromor sulphur and gypsum) and three levels of sulphur (20, 35 and 50 kg S ha-1) from both the sources along with control by adopting RBD with three replications. The result showed that yield attributes like plant height, bulb length, bulb diameter, neck length and average weight of bulb were increased significantly up to 35 kg S ha-1 and thereafter, declined in both the sources of sulphur. The maximum bulb yield was obtained with application of sulphur at 35 kg ha-1 and it was significantly superior to no sulphur and 20 kg S ha-1 and at par with 50 kg S ha-1 in both the sources of sulphur. Application of 35 kg S ha-1 produced an additional bulb yield of 3.8 t ha-1 in gypsum and 5.4 t ha-1 in gromorsulphur over control. The sulphur use efficiency of the crop showed an increasing trend up to 35 kg S ha-1 and thereafter decreased in both the sources. The highest net return and benefit cost ratio were achieved with the application of 35 kg S ha-1 in both the sources of sulphur. Comparing two sulphur sources, gromorsulphur resulted in higher growth, bulb yield, sulphur use efficiency and economics in comparison to gypsum.

This study has been able to determine the physiochemical properties, secondary and tertiary structure, and phylogenetic analysis of GAPDH among domestic animals under in silico platform. Eighteen nucleotide and protein sequence of GAPDH gene of different mammalian species were retrieved from National Centre for Biotechnology information (NCBI). The percentage of identity and similarity was done by Basic Local Alignment Search Tool (BLAST), physiochemical properties were analyzed by ExPASy”s ProtParam tool, the secondary and 3-D structure was predicted by GOR IV and Swiss modeling respectively. Phylogenetic analysis among the animals was done by Molecular Evolutionary Genetics Analysis. It was found that the percentage of identity and similarity among all animals were almost more than 90%. The physiochemical analysis showed this protein is very stable, hydrophilic and intracytoplasmic in nature. The secondary structure analysis showed that GAPDH has more number of random coil (49.85%) Extended strand (27.93%), alpha helix (22.22%) of the protein. The QMEAN Z score was found 0.33 under protein modeling which interfered that this protein is of comparable quality. The phylogenetic analysis of this gene showed that the highest time of divergence occurred between sheep and common chimpanzee but least time of divergence observed between killer whale and dolphin. So it can be concluded that the GAPDH gene is highly conserved along all animal species.

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Aim:The aim of this study is to evaluate and compare the pushout bond strengths of Resilon with two different sealers: Resilon/MetaSEAL (methacrylate based) and Resilon/AH Plus (an epoxy resin-based sealer).Materials and Methods:Forty single canal anterior teeth were decoronated at cementoenamel junction and standardized to 10 ± 1 mm length. Working length was determined followed by biomechanical preparation. Then, the specimens were randomly assigned into two groups of 20 teeth each based on the sealer used with Resilon. All canals were obturated using single-cone obturation technique. Root samples were prepared for pushout testing. The universal testing machine gave the debonding force for individual specimen. This was done for all the specimens.Statistical Analysis:This was done by using unpaired Student's t-test.Results:The roots filled with Resilon/MetaSEAL had higher bond strength (1.49 ± 0.09 MPa) compared to Resilon/AH Plus (0.90 ± 0.04 MPa) group. The difference in bond strength was statistically significant (P = 0.0000).Conclusion:Through this pushout bond strength test, it could be noted that MetaSEAL did appear to bond to the dentin and could be used as a potential endodontic sealer.