the effect of long-term oral taurine administration to rats on activity of lactate dehydrogenase (LDH), its isozyme content and activity in the whole blood, liver, thigh muscle, brain and testes tissues were studied in the present work. For this purpose male Wistar rats with body weight 190-220 g were randomly divided into three groups, they were orally administered drinking water (control group) or taurine solution 40 and 100 mg per kg of body weight ( groups I and II, respectively). the total lactate dehydrogenase activity was measured spectrophotometrically, the percentage content of isozymes was determined by electrophoresis in 7.5% poliacrylamide gel with further staining according to J. glucose metabolism in the organism and, possibly, regulate the activity of enzymes associated with glycolysis. Adding taurine to neurons previously incubated with MPP + , a substance that causes neurons degeneration, lead to increased lactate dehydrogenase activity and ATP re-synthesis [7]. Garbus. It was found that the total lactate dehydrogenase activity increased in all studied tissues. In testes of animals of both groups and in brain of group I animals, the total percentage contents of isozymes that are responsible for lactate production (LDH4+LDH5) increased. In liver of animals of both groups and in whole blood of group II animals, the total percentage content of isozymes that produce pyruvate (LDH1+LDH2) increased. In thigh muscle of both groups and in brain of group II animals the balance between LDH1+LDH2 and LDH4+LDH5 content did not differ fromSince lactate dehydrogenase is one of the markers of metabolic functional stability, the aim of the study was to investigate the effect of long-term oral administration of taurine on the total lactate dehydrogenase enzyme activity and the content of its isozymes in different rat tissues. materials and methodsMale Wistar rats were used in the experiments. The rats were 4.5 months old and had body weight of 190-220 g at the beginning of the experiment. Animal experiment was performed in accordance with European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes and the law of Ukraine On the protection of animals against Cruel treatment. Animals were randomly assigned to three groups: one control group and two experimental groups. Over a 28 day period, the animals were daily administered in the esophagus with: control groupdrin king water, group I -solution of taurine 40 mg/ kg, group II -solution of taurine 100 mg/kg. First dose was selected because it induced recovery of the nervous system in mice after administration of ethanol per os [8]. Second dose is one fiftieth of LD 50 and often used in studies of taurine detoxication effects [9][10][11]. The animals were decapitated on the 29 th day under light chloroform anesthesia and blood was collected in a tube with heparin. Liver, brain, testis and thigh muscle were isolated. After blood sampling, the number of erythrocytes was determined by colorimetry at 670 nm [12]. The h...
Rodenticides are a class of biocides whose primary purpose is to kill rodents. Rodenticides are divided by the mechanism of action into acute and chronic. In Ukraine, most of the registered drugs are of chronic action, as they are more effective against rodents. Bromаdiolone and brodifacoum are the main active substances of rodenticides with a chronic effect. Since these substances absorb well in the ultraviolet spectrum and their concentration in the preparation is low -0.005%, the quality control of chronic rodenticides is carried out, in most cases, by high-performance liquid chromatography. The purpose of the work was to analyze the quantitative content of active substances in solutions of bromаdiolone and brodifacoum and in ready-made rodenticides (grain, paste) during 2012–2022 and to establish possible reasons for non-compliance with the specification. The study was conducted using a Dionex Ultimate 3000 chromatograph and validated methods for determining the quantitative content of bromаdiolone and brodifacoum in rodenticides. Thirty-six samples of ready-made rodenticides and solutions for their preparation were analyzed. It was found that one third of the examined samples did not meet the requirements of regulatory documentation. Of the 22 ready-made baits for the destruction of rodents, 5 did not meet the specification. Since the active substance in the preparation itself is a little -0.005%, it can be assumed that these results are related to the violation of technological regulations in the production process. However, we found that out of 14 analyzed solutions for the production of ready-made rodenticides, in the form of processed grains or briquettes, 7 do not meet the requirements of the specification. This may be due to errors in the preparation of bromаdiolone and brodifacoum solutions, or to substances that do not correspond to what is stated in the quality certificate. Therefore, it is very important for manufacturing companies to carry out incoming control of raw materials, both substances and solutions for the preparation of ready-made rodenticides.
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