Activity and isozyme content of lactate dehydrogenase under long-term oral taurine administration to rats

Ostapiv, R. D.; Humenyuk, S. L.; Manko, V. V.

doi:10.15407/ubj87.04.054

Cited by 5 publications

(7 citation statements)

References 10 publications

(11 reference statements)

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“…By comparing LDH isoforms, we have revealed that in the DM group relative activity of LDH1 was decreased, while relative activity of LDH4 was increased. Decreased activities of LDH1 and LDH2 might indicate reduced formation of pyruvate [7] and insufficient supply in oxygen. Also, an increase in LDH4 activity may indicate that there is hypoxic condition in the body [7].…”

Section: Discussionmentioning

confidence: 99%

“…Decreased activities of LDH1 and LDH2 might indicate reduced formation of pyruvate [7] and insufficient supply in oxygen. Also, an increase in LDH4 activity may indicate that there is hypoxic condition in the body [7]. According to the previous study, our results probably mean that there was insufficient oxygen supply and therefore shift to anaerobic metabolism in diabetic rats' hearts.…”

Section: Discussionmentioning

confidence: 99%

“…LDH3 has the same affinity for both pyruvate and lactate. The last two isoforms dominate in the tissues where glycolysis predominates [7]. LDH, aspartate aminotransferase, and creatine kinase are cardiac marker enzymes which provide information about cardiomyocyte damage.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Folic Acid Administration on Cardiac Oxidative Stress and Cardiovascular Biomarkers in Diabetic Rats

Mutavdzin

Gopčević

Stanković³

et al. 2019

Oxidative Medicine and Cellular Longevity

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The aim of this study was to examine the effects of folic acid administration on the antioxidant enzyme (superoxide dismutase (SOD) and catalase (CAT)) activities, lactate and malate dehydrogenase (LDH and MDH) activities, and certain LDH and MDH isoform distribution in the cardiac tissue of diabetic Wistar male rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). There were five groups: C1—control (physiological saline 1 ml/kg, i.p. one day), C2—control with daily physiological saline treatment (1 ml/kg, i.p. 28 days), DM—diabetes mellitus (STZ 100 mg/kg in physiological saline, i.p. one day), FA—folic acid (5 mg/kg in physiological saline, i.p. 28 days), and DM+FA—diabetes mellitus and folic acid group (STZ 100 mg/kg in physiological saline, i.p. one day, and folic acid 5 mg/kg in physiological saline, i.p. 28 days). After four weeks, animal hearts were isolated for measurement of enzyme activities, as well as for histomorphometry analyses. An elevated glucose level and a decreased insulin level were obtained in the DM group. SOD, CAT, and MDH activities were elevated in the DM group, while there was no difference in LDH activity among the groups. In all tested groups, four LDH and three MDH isoforms were detected in the heart tissue, but with differences in their relative activities among the groups. Left ventricular cardiomyocyte transversal diameters were significantly smaller in both diabetic groups. Folic acid treatment of diabetic rats induced a reduced glucose level and reduced CAT, SOD, and MDH activities and alleviated the decrease in cardiomyocyte diameters. In conclusion, increased activities of antioxidant enzymes and MDH may be the consequence of oxidative stress caused by DM. Administration of the folic acid has a protective effect since it leads to reduction in glycemia and activities of the certain examined enzymes in the rats with experimentally induced DM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Effects of Folic Acid Administration on Cardiac Oxidative Stress and Cardiovascular Biomarkers in Diabetic Rats

Mutavdzin

Gopčević

Stanković³

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Moreover, lactate produced from glucose or glycogen in astrocytes is transferred to neurons that oxidize it into pyruvate to ensure an adequate energy supply [ 123 ]. On this basis, we suggest that the combination of zinc and taurine (Zn+IUI+Tau group) increased lactate and pyruvate flux between the astrocytes and neurons to modulate neuronal functions, such as excitability, plasticity, memory consolidation, and motor activity [ 123 – 125 ].…”

Section: Discussionmentioning

confidence: 99%

Taurine Increases Zinc Preconditioning-Induced Prevention of Nitrosative Stress, Metabolic Alterations, and Motor Deficits in Young Rats following Intrauterine Ischemia

Gonzalez-Vazquez

Aguilar-Peralta

Tomás-Sanchez

et al. 2021

Oxidative Medicine and Cellular Longevity

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Oxygen deprivation in newborns leads to hypoxic-ischemic encephalopathy, whose hallmarks are oxidative/nitrosative stress, energetic metabolism alterations, nutrient deficiency, and motor behavior disability. Zinc and taurine are known to protect against hypoxic-ischemic brain damage in adults and neonates. However, the combined effect of prophylactic zinc administration and therapeutic taurine treatment on intrauterine ischemia- (IUI-) induced cerebral damage remains unknown. The present work evaluated this issue in male pups subjected to transient IUI (10 min) at E17 and whose mothers received zinc from E1 to E16 and taurine from E17 to postnatal day 15 (PND15) via drinking water. We assessed motor alterations, nitrosative stress, lipid peroxidation, and the antioxidant system comprised of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Enzymes of neuronal energetic pathways, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH), were also evaluated. The hierarchization score of the protective effect of pharmacological strategies (HSPEPS) was used to select the most effective treatment. Compared with the IUI group, zinc, alone or combined with taurine, improved motor behavior and reduced nitrosative stress by increasing SOD, CAT, and GPx activities and decreasing the GSSG/GSH ratio in the cerebral cortex and hippocampus. Taurine alone increased the AST/ALT, LDH/ALT, and AST/LDH ratios in the cerebral cortex, showing improvement of the neural bioenergetics system. This result suggests that taurine improves pyruvate, lactate, and glutamate metabolism, thus decreasing IUI-caused cerebral damage and relieving motor behavior impairment. Our results showed that taurine alone or in combination with zinc provides neuroprotection in the IUI rat model.

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“…Treatment of rats with 1 % taurine solution for 15 days increased the content of TBA-active products and catalase activity in liver [16]. Per oral treatment for 28 days with taurine in doses 40 and 100 mg/kg increased lactate dehydrogenase activity and intensified mitochondrial respiration of hepatocytes [22,23]. However, lipid peroxidation processes were also increased that resulted in the increase of TBA content [24].…”

Section: Introductionmentioning

confidence: 99%

Influence of long-term per oral taurine treatment on activity of liver enzymes in mature rats

Ostapiv

Manko

2018

Biol. Stud.

Self Cite

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Taurine is sulfur-containing derivative of methionin and cysteine that plays a key role in glucose and lipid metabolism. Taurine regulates antioxidant defense system, membrane potential of mitochondria, and glycolysis. Thus, it might have a serious impact on cell metabolism in mammals. The aim of this work was to study the influence of long-term per oral taurine treatment (5, 10 and 20 mg/kg) on the antioxidant defense system, cholesterol content, activity of transaminases and lactate dehydrogenase in rat liver. To achieve that goal, mature Wistar rats (weight 140-160 g, age -4 months) were divided in four groups -control, rats which once a day during 28 days were injected drinking water in esophagus, and three experimental groups, that were injected taurine solution in concentrations: І -5 mg/kg, ІІ -10 mg/kg and ІІІ -20 mg/kg of body mass. After 28 days, rats were decapitated under light chloroform narcosis and liver mass was determined. In liver, content of cholesterol, TBA-active products and activity of antioxidant defense enzymes, transaminases, and lactate dehydrogenase were measured.Our results show that after a long-term per oral taurine treatment liver mass decreased by 27.5 % comparing to control group. Lactate dehydrogenase activity increased two times, while the activity of alanine aminotranferase and aspartate aminotransferase in mitochondrial fraction increased by 40-90 % in liver of animals of all experimental groups. Total activity of these enzymes in rat liver increased by 20-37 % in all experimental groups. Cholesterol content increased dy 32.4 % in rats of experimental group II. This may indicate intensification of liver metabolism. The content of TBA-active products in submitochondrial and mitochondrial fractions increased more than two times in rats of experimental groups II and III. The activity of superoxide dismutase in the submitochondrial fraction increased by 49.8 and 36.5 % in liver of that rats. The activation of superoxide dismutase and a rise in content of TBA-active products may suggest a rise of free oxygen radicals production and inability of antioxidant defense system. These results may indicate a negative influence of taurine in doses higher than 5 mg/kg, since the balance between antioxidant protection and lipid peroxidation processes was violated.

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Activity and isozyme content of lactate dehydrogenase under long-term oral taurine administration to rats

Cited by 5 publications

References 10 publications

The Effects of Folic Acid Administration on Cardiac Oxidative Stress and Cardiovascular Biomarkers in Diabetic Rats

The Effects of Folic Acid Administration on Cardiac Oxidative Stress and Cardiovascular Biomarkers in Diabetic Rats

Taurine Increases Zinc Preconditioning-Induced Prevention of Nitrosative Stress, Metabolic Alterations, and Motor Deficits in Young Rats following Intrauterine Ischemia

Influence of long-term per oral taurine treatment on activity of liver enzymes in mature rats

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