The Effects of Folic Acid Administration on Cardiac Oxidative Stress and Cardiovascular Biomarkers in Diabetic Rats

Abstract

The aim of this study was to examine the effects of folic acid administration on the antioxidant enzyme (superoxide dismutase (SOD) and catalase (CAT)) activities, lactate and malate dehydrogenase (LDH and MDH) activities, and certain LDH and MDH isoform distribution in the cardiac tissue of diabetic Wistar male rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). There were five groups: C1—control (physiological saline 1 ml/kg, i.p. one day), C2—control with daily physiological saline treatment (1 ml/kg, i.p. 28 days), DM—diabetes mellitus (STZ 100 mg/kg in physiological saline, i.p. one day), FA—folic acid (5 mg/kg in physiological saline, i.p. 28 days), and DM+FA—diabetes mellitus and folic acid group (STZ 100 mg/kg in physiological saline, i.p. one day, and folic acid 5 mg/kg in physiological saline, i.p. 28 days). After four weeks, animal hearts were isolated for measurement of enzyme activities, as well as for histomorphometry analyses. An elevated glucose level and a decreased insulin level were obtained in the DM group. SOD, CAT, and MDH activities were elevated in the DM group, while there was no difference in LDH activity among the groups. In all tested groups, four LDH and three MDH isoforms were detected in the heart tissue, but with differences in their relative activities among the groups. Left ventricular cardiomyocyte transversal diameters were significantly smaller in both diabetic groups. Folic acid treatment of diabetic rats induced a reduced glucose level and reduced CAT, SOD, and MDH activities and alleviated the decrease in cardiomyocyte diameters. In conclusion, increased activities of antioxidant enzymes and MDH may be the consequence of oxidative stress caused by DM. Administration of the folic acid has a protective effect since it leads to reduction in glycemia and activities of the certain examined enzymes in the rats with experimentally induced DM.

Syringic Acid Attenuates Cardiomyopathy in Streptozotocin-Induced Diabetic Rats

“…In the current study, elevated activity of cardiac CAT and SOD activities were determined in untreated diabetic rats. These results were similar to the results of other studies [ 32 – 35 ]. Conversely, some other reports revealed decreased CAT and SOD activities [ 36 – 38 ].…”

Hematological and renoprotective effects of folic acid and lentil extract in diclofenac sodium exposed rats

“…Oxidative stress markers were ameliorated by FA supplementation may be due to its direct effect on free-radical oxidation of LDL lipids (Lee et al, 2011). Also, FA has an antioxidant effect, it is reported that its administration decreases the production of ROS (Mutavdzin et al, 2019). The significant result of LE was due to its ability in restoring renal function by restoring antioxidants and preventing cellular damages.…”

Environmental Stress in Chickens and the Potential Effectiveness of Dietary Vitamin Supplementation

“…In quail, dietary supplementation of folic acid (2 mg/kg) alleviated the negative effects of CS on growth performance, lipid peroxidation, and serum concentrations of homocysteine (Sahin et al, 2003a). In mouse, administration of folic acid ameliorated the inhibitory effects of HS on the pre-implantation embryos development (Koyama et al, 2012), served a protective role by reducing the CAT, SOD, and glucose level (Padmanabhan et al, 2018;Mutavdzin et al, 2019). The ameliorative effects of vitamin B in chickens challenged with environmental stress are rarely studied.…”