Summary In order to investigate the pathophysiologica! significance of anti-endothelial cell antibody (AECA) in pre-eclampsia, the effects of AECA on endothelin-1 (ET-I) and prostaglandin I-, (PGI-,) release from cultured human umbilical vein endothelial cells (HUVEC) was evaluated. Serum samples were taken from 85 pre-eclamptic and 20 normal pregnant women. Anti-endothelial cell antibody was measured by ELISA using HUVEC. The release of ET-1 and 6-keto PGEl-a, a stable metabolite of PGI,, from HUVEC were evaluated after incubation with IgG-AECA-positive sera and IgG isolated from AECA-positive sera. The incidence of IgG-and IgM-AECA was 24.7 and R.2%, respectively. The release of ET-I in the medium containing IgG-AECA-positive sera was significantly greater than in the medium containing IgG-AECA-negative sera. There was signiticant correlation between the levels of IgG-AECA and the release of ET-1 from endothelial cells. The ET-1 relea.se by IgG isolated from AECA-positive sera was greater than that from AECAnegative .sera. However, the release of 6-keto PGFI-a by AECA-positive sera was not significantly different from that of AECA-negative sera. ]( is concluded that IgG-AECA in pre-eclampsia increases ET-I release from endothelial cells and that AECA may affect local vascular function in this disorder.
Anti-β2-GPI antibody-positive sera and IgG suppress the production of PlGF from JEG-3 cells. We suggest that the anti-β2-GPI antibodies may suppress PlGF production from trophoblasts and cause the failure of placenta formation and function.
summaryIn order to know the comlement activation, the changes of compliment were evaluated in recurrent abortion and pregnancy loss patients with antiphospholipid antibody (APA) positive. Serum samples were taken from 82 patients with more than 2 recurrent abortion patients and/or pregnancy loss. Fifty eight cases of 82 patients were APA and antinuclear antibody negative without autoimmune disease. Anticardiolipin antibody (ACA), anti CL/b2 GPI antibody (CL/b2 GPI) were measured using ELISA. Lupus anticoagulant (LAC) was measured using diluted RVVT. CH50 and C3 and C4 were measured as complements. The number of positive cases of ACA, CL/b2 GPI, LAC was 23, 9 and 5 cases, respectively.Results: The levels (mean±SD) of CH50, C3 and C4 in ACA positive and negative cases were 38.8±8.3 U/ml, 82.7±20.1 mg/dl, 18.5±5.7 mg/dl and 42.4±6.9 U/ml, 93.5±17.6 mg/dl, 21.1±4.6 mg/dl, respectively. The levels of CH50, C3 and C4 in positive cases were signiˆcantly lower than negatives. The levels of CH50, C3 and C4 in CL/b2 GPI and LAC positive cases were also signiˆcantly lower than negatives.Conclusion: The comlement activation was demonstrated in recurrent abortion and pregnancy loss with antiphospholipid antibody positive.
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