Gangliosides were extracted by various procedures from acetone powder prepared from grey matter of cattle brain. The mixtures of gangliosides, containing 2 4 2 6 % N-acetylneuraminic acid (NANA), were dissolved in chloroform-methanol. Upon addition of aqueous solutions of Caa+, the amount of gangliosides (and Caa+)passing into the lower phase of the two-phase solvent system was a linear function of the product of the initial concentrations of Ca2+ and ganglioside-NANA. It was also a function of the initial stoichiometric ratios of ganglioside-NANA to Caz+. In accordance with previous reports, no gross differences of composition between the initial mixture of gangliosides and the calcium-solubilized portion could be detected by glass-fibre chromatography.
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