Diazotrophic rhizobacteria, trigger and enhance plant growth as well as yield through various mechanisms, so their use can reduce the application frequency of chemical fertilizers. Indole-3-acetic acid (IAA), a most common natural auxin influences several physiological processes of the plant's health. The present study is aimed to optimize the conditions for IAA production, along with assay for plant growth promoting traits of Bacillus subtilis DR2 (KP455653), which is a diazotrophic Gram positive, rod bacterium, isolated from rhizosphere of road side weed, Eragrostis cynosuroides from Danapur, Patna, Bihar, India. The screening for IAA production was done in JNFbˉ broth with tryptophan (1 g.l-1) and without tryptophan at pH 5.8, 30±2 °C temperature and 48 h incubation. 137.81 µg.ml-1 and 100.26 µg.ml-1 IAA was produced in Trp + and Trpmedia, respectively. Under various optimized conditions, maximum IAA was produced at 96 h incubation (137.81 µg.ml-1), 35 °C temperature (141.92 µg.ml-1), pH 7 (158.79 µg.ml-1), mannitol as carbon (160.85 µg.ml-1) and ammonium sulfate as nitrogen (162.93 µg.ml-1) sources with tryptophan at final concentration of 1.2 µg.ml-1 (168.09 µg.ml-1), which enhanced the production by 1.2 fold. The findings suggest that B. subtilis DR2 is a potent organism to be used as biofertilizer. 

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.