Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

Abstract

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.

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“…They were incubated for 4-6 weeks at 27±2 °C temperature, 16/8 hour photoperiod and 50μmol-1 m -2 s -1 white daylight fluorescently illuminated at standard culture conditions [22]. For in vitro mass propagation of S. lycopersicum micro-shoots after germination and development stage, the micro-shoots were obtained with at least a binodal segment from each plant material under sterile conditions then, they were transferred to MS medium containing 1mgL -1 BA [23]. It was incubated for 4-6 weeks at standard culture conditions described above.…”