Alkaline phosphatase (ALP) belongs to hydrolase group of enzymes. It is responsible for removing phosphate groups from many types of molecules, including nucleotides and proteins. Cimetidine (trade name Tagamet) is an antagonist of histamine H2-receptor that inhibits the production of gastric acid. Cimetidine is used for the treatment of gastrointestinal diseases. In this study the inhibitory effect of cimetidine on mouse renal ALP activity was investigated. Our results showed that cimetidine can inhibit ALP by uncompetitive inhibition. In the absence of inhibitor the V(max) and K(m) of the enzyme were found to be 13.7 mmol/mg prot.min and 0.25 mM, respectively. Both the Vmax and Km of the enzyme decreased with increasing cimetidine concentrations (0- 1.2 mM). The Ki and IC(50) of cimetidine were determined to be about 0.5 mM and 0.52 mM, respectively.
In this work, a He atmospheric plasma jet was generated in stable bullet mode by using a dielectric barrier single electrode configuration. The plasma bullets were generated by four different excitation high-voltage waveforms and propagated through a dielectric barrier quartz tube. Corresponding charge, propagation speed and density were evaluated by current monitoring of the bullets, which were flowing through the travelling-bullet tube. It was revealed that positive bullets will be generated with higher current signals, charge, density and propagation speed by an excitation high-voltage waveform having a broader positive width and a greater portion of negative voltage (complex waveform). On the other hand, an excitation highvoltage with negative polarity initiates faster bullets than that of positive polarity. Last but not least, the optical emission spectroscopy results confirmed our electrical diagnostic results, and showed that more active and intense plasma is generated by the waveform with longer positive width and more negative portion.
Considering a piezo-plasma-like medium with hexagonal symmetry whose main symmetry axis is parallel to the z-axis and approximating it by an isotropic medium, the coupling of the elastic waves with plasma properties of the medium is studied. In this case, the coupled surface quasi-elasto-electromagnetic wave propagating on the interface of a piezoelectric with vacuum, making use of the mirror reflection model, is investigated. It is shown that the piezo-effect can result in the plasma damping of the elastic oscillations.
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