Taxonomic relationships among 22 isolates of nine species of the wood‐rotting fungus, Polyporus, were examined by starch gel electrophoresis, coupled with histochemical staining procedures. Crude extracts of 21‐day cultures grown on malt extract agar were subjected to electrophoresis for 3 hr at a constant current of 25 ma. Esterase, peroxidase, catechol oxidase, galactose dehydrogenase, acid phosphatase, and alkaline phosphatase banding patterns were analyzed. With each enzyme, the bands for each isolate were compared with those for other isolates of the same species and for isolates of other species. A closer relationship was found in the banding patterns for different isolates of a single species than among isolates of different species. This technique may prove valuable for the evaluation of taxonomic differences among these wood‐rotting fungi.
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