Starch Gel Electrophoresis of Enzymes From Nine Species of Polyporus

Abstract

Taxonomic relationships among 22 isolates of nine species of the wood‐rotting fungus, Polyporus, were examined by starch gel electrophoresis, coupled with histochemical staining procedures. Crude extracts of 21‐day cultures grown on malt extract agar were subjected to electrophoresis for 3 hr at a constant current of 25 ma. Esterase, peroxidase, catechol oxidase, galactose dehydrogenase, acid phosphatase, and alkaline phosphatase banding patterns were analyzed. With each enzyme, the bands for each isolate were compared with those for other isolates of the same species and for isolates of other species. A closer relationship was found in the banding patterns for different isolates of a single species than among isolates of different species. This technique may prove valuable for the evaluation of taxonomic differences among these wood‐rotting fungi.

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

“…It is known that isozyme variation in some fungi is influenced by growth substrate (Shannon et al 1973), by age of the fungus, and by the enzyme systems studied (Franke 1973). In our study, some of these effects were avoided or reduced by culturing all the isolates in the same growth medium and conditions, by comparing differing ages of the cultures, and by the random elements involved in selection of the enzymes.…”

Taxonomic relationships within the fungal genus Pleurotus as determined by isoelectric focusing analysis of enzyme patterns

“…Studies utilizing enzyme electrophoresis have attempted to discriminate among species in genera such as Polyporus (Shannon et al, 1973), Fomes (Harris et al, 1974), Monilinia (Willets et al, 1977), Botrytis (Backhouse et al, 1984), Agaricus (Kerrigan & Ross, 1988) and Phytophthora (Oudemans & Coffey, 1991) or to characterize Culture media and conditions. The mycelial cultures were maintained and propagated on complete yeast medium (CYM) (Raper etal., 1972), comprising (g 1-l): glucose (20); bactopeptone (2); yeast extract (2); MgSO,.…”

Ferramentas Moleculares Para Caracterização De Candida Albicans (Robin) Berkhout (1923) Em Estudos Epidemiológicos

“…Os padrões de bandas eletroforéticas isoenzimáticas são freqüentemente previsíveis, uma vez que dependem das condições genéticas e nucleares de cada organismo. No entanto, vários micologistas restringiram as interpretações dos resultados eletroforéticos à mera contagem de bandas (65,66,67,68,69). A interpretação genética, quando possível, fornece muitas informações adicionais acerca da condição nuclear, genética e taxonomia de um grupo de organismo (70,71,72).…”