The anthocyanins from Ajuga pyramidalis Metallica Crispa cell
cultures were characterized by a
combination of HPLC, chemical hydrolysis, MS, and 1H NMR.
Chromatographic analysis of the in
vivo and in vitro samples showed the same suite of anthocyanins and
relative composition. Chemical
hydrolysis and mass spectral analysis of the major pigment from
suspension cultures showed the
presence of cyanidin, three glucoses, two ferulic acids, and malonic
acid. The structure of the major
pigment in the suspension cultures was determined to be
3-O-(6-O(E)-ferulyl)-2-O-[(6-O(E)-ferulyl)-β-d-glucopyranosyl-β-d-glucopyranosyl]-5-O-(6-O-malonyl)-β-d-glucopyranosylcyanidin.
The anthocyanins in the in vitro extract were more stable toward light compared
to those in the in vivo extract.
The initial degradation of the major pigment both from in vitro
and in vivo sources was mainly due
to demalonylation.
Keywords: Ajuga; anthocyanins; cell culture;
stability-anthocyanins
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