The detection of a new SSCP polymorphism within the promoter of the bovine alpha SI casein gene is described. The fragment of alpha SI casein gene promoter (218 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 118 randomly chosen cows (Black-and-White) and 124 bulls (Black-and-White), we found two SSCP patterns, labelled R2 and R3, with respective frequencies of 0.05 and 0.95. The detected SSCP polymorphism may be useful in searching for associations between different SSCP patterns and gene expression, especially milk protein percentage and protein yield.
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