SSCP polymorphism within a promoter of the
bovine alpha S1 casein gene

Kamiński, Stanisław; Jozefowicz, S

doi:10.22358/jafs/68030/2000

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…One portion of semen was used to isolate genomic DNA by the MasterPure DNA Purification Kit (Epicentre). DNA was used to amplify 6 PCR products, namely: a 208 bp fragment of LGB promoter, designated LGB-R (Kaminski and Zabolewicz, 1998), a 240 bp fragment of LGB exon IV, designated LGB (Kaminski and Zabolewicz, 1997), a 214 bp fragment of CASK promoter, designated CASK-R (Kaminski, 1996), a 273 bp fragment of CASK exon IV-intron IV, designated CASK (Kaminski, 1998), a 483 bp fragment of LALBA 5' flanking region, designated LALBA (Kaminski, 1999) and a 218 bp fragment of CSN1S1 promoter, designated CSN1S1 (Kaminski and Jozefowicz, 2000). The breeding values of bulls were collected from the official ranking list of proven bulls published bi-annually by the National Animal Breeding Centre in Warsaw (Poland).…”

Section: Methodsmentioning

confidence: 99%

Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls

Kamiński¹,

Rymkiewicz-Schymczyk²,

Wójcik³

et al. 2002

J. Anim. Feed Sci.

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One hundred forty three proven Polish Black-and-White bulls were genotyped for 6 polymorphic sites located within 4 milk protein genes (LGB-R promoter, LGB exon IV, CASK-R promoter, CASK exon IV, LALBA 5' flanking region and CSN1S1 promoter). All bulls were divided into groups, including animals with the same genotypes, intragenic haplotypes and combinations of genotypes. For each group, the arithmetic mean and standard deviation for Type Production Index (TPI) and Predicted Transmitting Ability (PTA) (milk kg, protein kg, protein %, fat kg, fat %) were calculated. These data were then used for analysis of variance to find possible associations between identified polymorphic sites and TPI and PTA values.

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Section: Methodsmentioning

confidence: 99%

Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls

Kamiński¹,

Rymkiewicz-Schymczyk²,

Wójcik³

et al. 2002

J. Anim. Feed Sci.

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“…Polymorphism within the 5'-flanking region of the bovine b-lactoglobulin gene (b-lgPTU) was studied by Kamin u ski and Zabolewicz [46] in the Polish Black-andWhite dairy cattle, in which the two predominant b-lactoglobulin variants are also A and B. Moreover, within the promoter region (b-lgP) of the gene, several point mutations were found by SSCP analysis.…”

Section: B-lactoglobulinmentioning

confidence: 99%

The impact of genetic polymorphisms on the protein composition of ruminant milks

et al. 2002

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-The purpose of this review is to give an overview of our current knowledge on the polymorphisms occurring in genes coding for milk proteins and responsible for quantitative variability in their expression, thus influencing the protein composition of livestock ruminant milk. The overall genomic organisation of the 6 main ruminant milk protein genes: a-lactalbumin, b-lactoglobulin and the four caseins (a s1 , a s2 , b and k), their chromosomal location and their expression pattern are first summarised before presenting general mechanisms controlling gene expression both at the transcriptional and the post-transcriptional levels. Polymorphisms found in cis-regulatory elements, mainly within the 5'-flanking region of the genes encoding b-lactoglobulin and a s1 -and a s2 -caseins, have been found, in cattle, to influence their transcription rate. In addition, polymorphisms found in the transcription unit, within intron as well as exon sequences, have been shown to be responsible for defects in the processing of primary transcripts and/or the export of messenger RNA to the cytoplasm. Mutations responsible for the occurrence of premature stop codons in a s1 -and b-casein mRNAs have been shown to be associated both with a decrease in the level of the relevant transcripts and the existence of multiple forms of messengers due to alternative splicing (exon skipping, usage of cryptic splice sites). Such a situation, well-exemplified by the gene encoding a s1 -casein in the goat, may have dramatic biological consequences (secretion pathway, casein micelle structure, fat content, etc.) by modifying the message and accordingly the primary structure of the protein as well as its expression. Since some of these polymorphisms dramatically affect technological properties of milk, including cheese yields and organoleptic characteristics, methods mainly based on the PCR technique have been designed and applied in selection and breeding programmes to improve milk protein quality.gene expression / milk protein / genetic polymorphism / ruminants Reprod. Nutr. Dev. 42 (2002) 433-459 433

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SSCP polymorphism within a promoter of the bovine alpha S1 casein gene

Cited by 2 publications

References 6 publications

Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls

Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls

The impact of genetic polymorphisms on the protein composition of ruminant milks

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