Introduction. Vinyl chloride, which has a polytropic effect on the human body is the main harmful substance in the production of vinyl chloride and polyvinyl chloride. The aim of the study was to analyze the dynamics (after 5 years) of the cytokine profile and autoimmune response against the proteins of the nervous tissue in workers with continued chronic exposure to toxicants. Material and methods. The paper presents the results of a laboratory immunological examination of those working in the production of vinyl chloride with an average level of occupational risk caused by exposure to vinyl chloride and 1,2-dichloroethane. Results. The character of cytokine regulation disorders in a primary examination in healthy workers and persons with initial manifestations of neurointoxication with vinyl chloride is characterized by a rise in proinflammatory IL-1β and IL-4 with a simultaneous decrease in IL-2 and TNFα, and a more pronounced hyperproduction IL-1β against the background of a decline in IL-4 and IL-2. At repeated examination 5 years after the increase of immunity intensity in healthy workers and disbalance in the system of pro - antiinflammatory cytokines in persons with initial manifestations of neurointoxication was registered, which determines the different degree of the manifestation of compensatory and protective responses that do not allow the full implementation of protective mechanisms in the case of chronic exposure. Discussion. A more pronounced decrease in immunoreactivity relative to the proteins of the nervous tissue in workers with initial manifestations of neurointoxication in the dynamics of chronic effects of production factors is shown. The results obtained made it possible to identify informative biomarkers (IL-1β, TNFα, INFγ, IL-4, S-100, NF-200, TMP) that allow us evaluating the degree of the expression of compensatory-protective responses of the immune response and can be used to monitor the development of the pathological process. Conclusion. Identification of the leading immunopathogenetic factors of neurological deficiency can be the basis for elaborating a method for predicting the individual risk of developing professional neurointoxication.
Objectives: The aim of this work is to perform comparative estimation of cytokines levels in chlorinated hydrocarbons and metallic mercury exposure in employees in the dynamics of neurologic disorders formation. Material and Methods: The contents of cytokines IL-1β, IL-2, IL-4, IL-6, TNF-α, INF-γ were determined in blood sera using the method of hardphasic immunoferment analysis. The significance of different average values was assessed using the parametric and non-parametric criteriaStudent (in normal distribution) and Mann-Whitney tests taking into account the Bonferonni correction (non-difference from normal distribution). Results: It was shown that, a number of inflammation mediators with the dominance, depending on the expositional toxicant and expression of neurological deficiency, take part in the neurointoxication development. Healthy employees show pro-inflammatory responses with different expression degree, which dominate in the immune regulation processes regardless of the expositional factors (metallic mercury vapors and chlorinated hydrocarbons). Conclusions: The production intensity and interconnection between the pro-and anti-inflammatory cytokines may change in the occupational injuries of the nervous system development process. The decrease in the serum concentrations of cytokines along with the increase of clinical manifestation severity may prove dysregulation of the immune system, which promotes maintaining of pathological process and progradient process of neurointoxication. The most obvious is the imbalance of cytokines in the employees exposed to metallic mercury (in all the examined groups) that increases neurointoxication in the distant period.
The prolonged impact of industrial vibration on workers leads to the development of a vibrational disease (VB), which occupies a leading position in the structure of occupational pathology. VB from the impact of local vibration is a chronic occupational disease characterized by a predominant lesion of the nervous, vascular system and musculoskeletal system of the upper and lower extremities. One of the real ways to reduce the incidence is the early detection of the negative impact of vibration on the body of workers. In this regard, cytokines and heat shock proteins (HSP70) can be early and sensitive indicators that reflect the severity of health disorders from exposure to vibration. The aim of the study was to study changes in the content of pro- and antiinflammatory cytokines, extracellular HSP70 and their relationship in patients with VB. In the immunological study included 43 male patients with a diagnosis of VB. The criteria for inclusion in this group were: a verified diagnosis, written informed consent to participate in the study, the harmful effects of local vibration in the workplace. According to the data of hygienic control, the working conditions of workers in dangerous occupations by vibration belong to the 4 (dangerous) class due to intensive local vibration. The content of cytokines: IL-1β, IL-8, IL-10, TNFα and HSP70 in the serum of patients was determined by enzyme immunoassay. The blood sampling for the study was taken from patients only once on admission to the hospital before the treatment. Statistical processing of data was carried out with the help of packages of application programs Statistica for Windows 6.0 and Microsoft Excel. It was established that the development of VB disease is accompanied by imbalance of the cytokine profile, characterized by a decrease in the levels of IL-1β, IL-10 and an increase in IL-8. The inhibition of production of IL-1β and IL-10 is a consequence of the chronic process that has developed in the body of the patients examined. And the revealed decrease in extracellular concentration of HSP70 in comparison with practically healthy people can be caused by the accumulation of it inside the cell or on its surface. Correlation analysis revealed the relationship between a decrease in HSP70 and an increase in IL-1β, and a decrease in IL-10 levels. Synthesis of HSP is an intracellular defense mechanism that prevents cell damage by activating the synthesis and secretion of pro-inflammatory cytokines. The obtained relationships between cytokines and HSP70 testify to the involvement of HSP70 in immunoinflammatory processes in VB. The revealed changes contribute to the chronic inflammatory process and justify the progressive course of the VB.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.