High efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform starting material is proposed. It speeds up the breeding process two to threefold. The identification of haploid regenerants with sterile cytoplasm in initial populations is of great theoretical and practical importance for breeding, as it facilitates the production of homozygous lines with cytoplasmic male sterility and high-performance hybrids on sterile basis. As shown by molecular analysis, a single-nucleotide polymorphism never reported hitherto is present in the mitochondrial genome of the haploid plant regenerants. It allows identification of microclones as fertile and sterile forms. It has been found that DNA markers of the sugar beet mitochondrial genome belonging to the TR minisatellite family (TR1 and TR3) enable reliable enough identification of haploid microclonal plants as MSor O-type forms. Fragments of 1000 bp in length have been detected in monogenic forms in the analysis of 11 sugar beet plants cultured in vitro by PCR with the OP-S4 random RAPD primer. Testing of the OP-S4 marker’s being in the same linkage group as the genes responsible for expression of the economically valuable trait monogermity demonstrates its relative reliability. By the proposed method, dihaploid lines (DH) of the male-sterile form and the О-type sterility maintainer of the RMS 120 sugar beet hybrid have been obtained in in vitro culture. These lines are highly uniform in biomorphological traits, as proven under field conditions.
Data on the biological activities of compounds containing m-dinitroaromatic (m-DAr) moieties were presented and led to the conclusion that including this moiety in the structures of new biologically active compounds was promising. It was established that one nitro group in m-DAr could be replaced by trifluoromethyl without losing the biological activity of the obtained analogs.Biologically active compounds with m-dinitroaromatic (m-DAr) moieties and examples of their use in medicine, veterinary medicine, and agriculture were reviewed. Compounds with m-DAr moieties were historically first used broadly as herbicides, fungicides, insecticides, and acaricides. Information about their activities was published in several monographs [1 -4]. 2,4-Dinitrophenols (I) were some of the first m-DAr compounds to find practical application as weed killers (Table 1).
Based on multi-location tests with different edaphic and climatic conditions and process approaches to the cultivation of field crops (Oryol, Samara, Nizhny Novgorod, Voronezh), the article dwells on the algorithm of a systemic approach to studying the individual characteristics of 17 varieties and samples of winter wheat, and shows the principles of their selection to form a single system for the purpose of stabilising the production of high-quality grain. Ecological approaches to the assessment of individual characteristics of different wheat varieties allowed identifying bottleneck issues related to the implementation of their adaptive potential, behaviour in the single system of agrobiogeocenosis, specifying the influence of the genotype–environment interaction on the manifestation of individual characteristics. The understanding of these issues makes it possible to develop a process sequence beneficial for the formation of genotypes with high productivity. Following the analysis, the breeds Leonida, Grom and Nemchinovskaya 85 wheat varieties were identified as promising for production in Central Russia. They showed high productivity and proved to be close in terms of sensitivity to growing conditions. In addition, a new wheat variety, Nemchinovskaya 85, demonstrated high quality of grain product. Izumrud Dubovitskogo and DF2020 varieties were distinguished by a high level of adaptive properties, expressed as their superiority in severe conditions of the Volga region and regions with a low level of soil moisture during grain filling. The study also suggests high processability and narrow limits of ecosystem boundaries when using Stork and Sineva wheat varieties in production, which most fully implemented their potential only in the system of intensive cultivation technologies with moderate to increased soil-moisture regimen in the second half of the vegetation season.
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