OBJECTIVE: To investigate the long-term effects of changes in dietary carbohydrateafat ratio and simple vs complex carbohydrates. DESIGN: Randomized controlled multicentre trial (CARMEN), in which subjects were allocated for 6 months either to a seasonal control group (no intervention) or to one of three experimental groups: a control diet group (dietary intervention typical of the average national intake); a low-fat high simple carbohydrate group; or a low-fat high complex carbohydrate group. SUBJECTS: Three hundred and ninety eight moderately obese adults. MEASUREMENTS: The change in body weight was the primary outcome; changes in body composition and blood lipids were secondary outcomes. RESULTS: Body weight loss in the low-fat high simple carbohydrate and low-fat high complex carbohydrate groups was 0.9 kg (P`0.05) and 1.8 kg (P`0.001), while the control diet and seasonal control groups gained weight (0.8 and 0.1 kg, NS). Fat mass changed by 7 1.3 kg (P`0.01), 7 1.8 kg (P`0.001) and 0.6 kg (NS) in the low-fat high simple carbohydrate, low-fat high complex carbohydrate and control diet groups, respectively. Changes in blood lipids did not differ signi®cantly between the dietary treatment groups. CONCLUSION: Our ®ndings suggest that reduction of fat intake results in a modest but signi®cant reduction in body weight and body fatness. The concomitant increase in either simple or complex carbohydrates did not indicate signi®cant differences in weight change. No adverse effects on blood lipids were observed. These ®ndings underline the importance of this dietary change and its potential impact on the public health implications of obesity.
Although it has been thirty years since publication of one of the most influential papers on the value of assessing sperm DNA damage, andrologists have yet to reach a general consensus about how to apply this seminal parameter to improve or predict reproductive outcomes. Studies that have attempted to establish a causal relationship between sperm DNA damage and pregnancy success have often resulted in conflicting findings, eroding the practitioner's confidence to incorporate this phenomenon into their appraisal of fertility. In this review we have identified and answered ten important unresolved questions commonly asked by andrologists with respect to the relationship between sperm DNA damage and fertility. We answer questions ranging from a basic comprehension of biological mechanisms and external factors that contribute to increased levels of sperm DNA damage in the ejaculate to what type of DNA lesions we might be expect to occur and what are some of the consequences of DNA damage on early embryonic development. We also address some of the fundamental technical issues associated with the most appropriate measurement of sperm DNA damage and the need to attenuate the confounding impacts of iatrogenic damage. We conclude by asking whether it is possible to reduce elevated levels of sperm DNA damage therapeutically.
Two independent data files from the breeding herd of Iowa State University and six North Carolina herds were used to examine relationships between yield traits and mtDNA polymorphism. Maternal lineages were established by tracing ancestry of cows to founder females in the herd book of the Holstein Association. Data from Iowa State University were 1476 records from 602 cows from 29 maternal lineages. The nucleotides of mtDNA encoding rRNA were sequenced. Eleven sites of polymorphism were found. An animal model for gene substitution was used to examine the relationship between sequence differences and yield traits. Traits analyzed were mature equivalent yield of milk, fat, SNF, and milk energy as well as concentrations of fat, SNF, and milk energy. Effects of sequence differences were significant for most traits. Sequence information from the D-loop was available for 12 lineages from North Carolina. The effect of polymorphism at 4 sites was examined using 1472 records from 668 cows. Traits measured were the same, except that protein replaced SNF. No significant relationships existed between any of the traits and D-loop polymorphism, but results suggested that an association might exist between polymorphism and concentrations of milk yield, fat percentage, and energy. Whenever a significant relationship was detected, the effect of mutation (rare genotype) was detrimental.
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