Dynamics of sperm DNA fragmentation in domestic animals

López‐Fernández, Carmen; Crespo, F.; Arroyo, F.; Fernández, José Luis Fernández; Arana, P.; Johnston, S. D.; Gosálvez, Jaime

doi:10.1016/j.theriogenology.2007.08.029

Cited by 121 publications

(102 citation statements)

References 43 publications

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“…Similar results have been reported in previous research in boars (Boe-Hansen et al, 2005;De Ambrogi et al, 2006;Enciso et al, 2006) and in horses (López-Fernández et al, 2007).…”

Section: Discussionsupporting

confidence: 92%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

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-The objective of this study was to evaluate the effects of season, natural photoperiod, and room temperature at the housing facility on boar semen characteristics in Uruguay (34º66'S; 56º29'W). For this purpose, 117 ejaculates, obtained from eight adult males collected through 12 consecutive months, were assessed for sperm viability, DNA integrity, abnormalities (total, primary, and secondary), ejaculate volume, and sperm concentration. Viability, total and primary abnormalities, volume, and sperm concentration were affected by season. Sperm viability, volume, and sperm concentration were affected by natural photoperiod. In general, autumn and the decreasing photoperiod had a negative impact on most of the semen characteristics, except for volume. Housing temperature did not affect semen characteristics. In boars living in temperate climates, semen quality is negatively affected during autumn and is related to photoperiod changes; however, the effects of temperature changes in housingdo not affect these seminal characteristics. In this scenario, seasonal differences in semen quality may have a negative effect on sow fertilization. Consequently, semen quality control especially during autumn is imperative for the best boar selection to be used for insemination purposes. Seasonal differences in semen quality may have a negative effect on sow reproductive performance. This issue will be addressed in a future investigation.

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“…Similar results have been reported in previous research in boars (Boe-Hansen et al, 2005;De Ambrogi et al, 2006;Enciso et al, 2006) and in horses (López-Fernández et al, 2007).…”

Section: Discussionsupporting

confidence: 92%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

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“…While the positive effect of colloidal centrifugation on seminal characteristics of stallion sperm has also been demonstrated in previous studies (Johannisson et al, 2009;Morrell et al, 2010b), we have demonstrated that this improvement can be further enhanced if the semen sample is fractionated on collection. Our observation of a reduction in rSDF-T6 following colloidal centrifugation is reported here for the first time in the stallion and is consistent with our other studies noting a possible association between slower DNA fragmentation dynamics and greater sperm viability in the female genital tract (López-Fernández et al, 2007;Cortés-Gutiérrez et al, 2008;Crespo et al, 2013).…”

Section: Concsupporting

confidence: 93%

“…Sperm DNA fragmentation (SDF) was assessed using the Halomax kit® (Halotech SL, Madrid, Spain). This methodology is based on the sperm chromatin dispersion test (SCD), the details of which have previously been described in a range of mammalian species, and which had already been validated for equine semen by our group (López-Fernández et al, 2007). Sperm DNA longevity in the different fractions was calculated after a dynamic SDF assessment protocol which was designed to emulate conditions of sperm physiology once inside female reproductive tract.…”

mentioning

confidence: 99%

Short communication. Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation

Crespo¹,

Gosálvez²,

Johnston³

et al. 2015

Span. j. agric. res.

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This study investigated the possible additive benefit of ejaculate fractionation and colloidal centrifugation on stallion sperm quality. Using an open-end artificial vagina, the sperm-rich fraction (FRAC-1) was separated from the rest of the ejaculate (FRAC-2) and a third sperm sample representing the combined ejaculate was reconstituted post-ejaculation (RAW). Each semen sample was processed for colloidal centrifugation. The percentage of abnormal spermatozoa was 17.8 ± 7.0% in RAW and 14.6 ± 9.5% in FRAC-1 but decreased to 11.4 ± 4.7% and 9.6 ± 6.9% respectively, after colloidal centrifugation. A sperm DNA fragmentation index of 10.9 ± 5.1% was observed in RAW and 7.5 ± 2.4% in FRAC-1 semen collected with the AV but this decreased to 7.8 ± 2.8% and 5.2 ± 2.3% after colloidal centrifugation. The rate of increase in sperm DNA fragmentation during the first 6 h of incubation at 37 ºC was 1.8 ± 0.9% per hour in RAW semen and 2.0 ± 2.0% per hour in FRAC-1 but this significantly decreased to 1.3 ± 1.4% and 0.9 ± 0.8% respectively after colloidal centrifugation. While stallion seminal characteristics can be improved using colloidal centrifugation, further enhancement is possible if the ejaculate is initially fractionated.Additional key words: Equus caballus; ejaculate fractionation; colloidal centrifugation; sperm DNA fragmentation; DNA longevity; SCD.Abbreviations used: FRAC-1 (first jet, sperm-rich fraction); FRAC-2 (rest of the ejaculate); RAW (combined ejaculate reconstituted post-ejaculation); ROS (reactive oxygen species); rSDF (rate of increase in sperm DNA fragmentation); SCD (sperm chromatin dispersion), SDF (sperm DNA fragmentation).

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“…The major advantage of this method over others is that each test requires very small numbers of sperm; moreover, the test is very cost efficient. This has enabled the development of a novel approach to DNA fragmentation studies, whereby the semen samples are incubated over a period of 24-48 h, and the dynamics of DNA fragmentation are calculated from repeated tests rather then a single, one-off test as typically performed by SCSA or TUNEL (75,76).…”

Section: Chromatin Defectsmentioning

confidence: 99%

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

Petrunkina

Harrison²

2011

Cytometry Pt A

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Cytometric methodologies are becoming increasingly important in veterinary andrology as means of assessing sperm function. However, as yet, flow cytometric techniques in veterinary andrology have not kept up in sophistication with those in other areas of biology and medicine. In this brief review, we consider the present state of cytometry in andrological procedures for evaluating the fertility of domestic animal sires. We outline the aspects of sperm physiology, paying particular attention to the changes that take place during the process known as capacitation, which prepares the sperm for interaction with the egg. We then examine briefly but critically the cytometric techniques that are currently in commercial use or are being established in research laboratories for testing sperm characteristics. Current limitations and potential developments in semen assessment are discussed. Recent research knowledge offers possibilities for applying more subtle flow cytometric approaches to distinguish different levels of fertilizing potential in semen samples. For example, linking field fertility data to multiparametric kinetic studies of sperm capacitational changes rather than ''single parameter-single time point'' estimations may reveal that slower rather than rapid changes indicate high fertility. Moreover, the development of multicolor flow cytometric procedures as a means of evaluating multiple functional parameters in individual cells would reduce the uncertainties always inherent in predicting fertility from in vitro sperm evaluation tests. ' 2011 International Society for Advancement of Cytometry Key terms fertility; sperm evaluation; heterogeneity; subpopulations; multicolour cytometry THE main focus of an andrological assessment in veterinary medicine is to ascertain the fertility of a potential sire. Although the most obvious and indeed frequently used general veterinary test is to quantify the number of offspring born to the sire, this is a lengthy and very expensive process. For ''accuracy,'' many inseminations must be carried out, and results must await gestation and birth. Obviously, therefore, development of rapid tests that can be carried out in the laboratory has for long been important. However, as research into the reproductive process has advanced, it has become clear that the life of the fertilizing sperm between its release from the testis and its fusion with the egg is far from simple. Moreover, the population of sperm ejaculated into the female has been revealed as being heterogeneous in functional ability, even if morphologically normal [c.f. reviews (1,2)]. As a result of these findings, tests are being developed to examine functional ability within a heterogeneously responding population (3-5).Given that a normal male ejaculate contains many millions of individual sperm cells, it is natural that these tests are based on cytometry. In this short review, we will outline and comment upon a range of modern and still-developing cytometric methodologies in use or potentially useful for sperm...

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Dynamics of sperm DNA fragmentation in domestic animals

Cited by 121 publications

References 43 publications

Seasonal variation in sperm characteristics of boars in southern Uruguay

Seasonal variation in sperm characteristics of boars in southern Uruguay

Short communication. Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

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