Problem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic variation is important to determine the genetic relationship between FOC isolates from different banana cultivars. Approach: Two PCRbased methods, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and restriction analysis of the Internal Transcribed Spacer and 5.8S regions (ITS+5.8S regions) were used to characterize Fusarium Oxysporum F. sp. Cubense (FOC) isolates from different banana cultivars. The genetic relationship of the FOC isolates were analyzed using Un-weighted Pair-Group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient. Results: Restriction patterns of the ITS+5.8S regions using nine restriction enzymes namely, Alu I, Eco RI, Eco 88I, Bsu RI, Bsu 15I, Hin fI, Hin 6I, Msp I and Taq I and ERIC-PCR showed low variation among the FOC isolates studied, indicating close relationship among the isolates. Unweighted Pair-group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient showed that the FOC isolates were grouped into two main clusters with similarity value of 41.4-100% in PCR-RFLP of ITS + 5.8S and 45-100% similarity based on ERIC-PCR analysis, respectively. Cluster analysis of the combined data also showed that the FOC isolates were grouped into two clusters, sharing 42.9-100% similarity. Conclusion/Recommendations: The results of the present study indicate that the FOC isolates were closely related regardless of banana cultivars and location.
The asymmetric unit of the title compound, {[Na(C6H12O6)2]Cl}n, contains six glucose molecules, three Na+ ions and three Cl− ions, i.e. three independent {[Na(C6H12O6)2]Cl} units. Each of these units forms polymeric chains along the c axis. Each Na+ ion is surrounded by six O atoms from four glucose molecules, forming a distorted octahedral geometry. All glucose molecules adopt chair conformations. The constituent units are linked into a three-dimensional framework by O—H⋯Cl and O—H⋯O hydrogen bonds, utilizing all the O—H groups.
Fusarium species were isolated from 12 cultivated soil planted with different crops in Penang. A total of 42 Fusarium isolates were recovered in which four Fusarium species were identified namely, F. solani, F. semitectum, F. equiseti and F. oxysporum. The most prevalent Fusarium species recovered was F. solani (84%), followed by F. semitectum (7%), F. equiseti (7%) and F. oxysporum (2%). The present study showed that Fusarium populations are diverse within cultivated soils and could be potential inoculum to infect certain agriculture crops.
F. oxysporum, F. proliferatum and F. solani isolates were recovered from root and stem rot of orchid. The isolates were placed into vegetative compatibility group (VCG) based on pairing of complementary mutants. From complementation tests, all F. solani isolates did not form heterokaryons and was therefore heterokaryon self-incompatible. For both F.oxysporum and F. proliferatum isolates, four VCG were identified. The results suggest that variation exist among the F. oxysporum and F. proliferatum isolates from root and stem rot of orchid.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.