Identification and Pathogenicity of Fusarium Species Associated with Root Rot and Stem Rot of Dendrobium

Zakaria, Latiffah; Hayati, Mardhiah; Baharuddin, S.; Maziah, Z.

doi:10.3923/ajppaj.2009.14.21

Cited by 27 publications

(25 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, F. solani could not be re-isolated from tissue expressing limited symptoms. Although F. solani has been shown in other studies to cause disease in Cymbidium (Benyon et al, 1996;Ichikawa & Saito, 1998;Lee et al, 2002) and Dendrobium (Latiffah et al, 2008(Latiffah et al, , 2009, there are no data to demonstrate pathogenicity of F. solani from these orchids to Phalaenopsis. Thus, the host ranges of F. solani that attack Cymbidium and Dendrobium are not clear.…”

Section: Discussionmentioning

confidence: 94%

“…, 1996; Ichikawa & Saito, 1998; Lee et al. , 2002) and Dendrobium (Latiffah et al. , 2008, 2009), there are no data to demonstrate pathogenicity of F. solani from these orchids to Phalaenopsis .…”

Section: Discussionmentioning

confidence: 99%

“…Fusarium solani has been reported as the pathogen causing leaf yellowing or root rot of Phalaenopsis (Morita et al, 1992;Kim et al, 2002;Lin et al, 2007;Chen et al, , 2009, dry rot or root rot in Cymbidium (Benyon et al, 1996;Ichikawa & Saito, 1998;Lee et al, 2002) and stem rot or root rot of Dendrobium (Latiffah et al, 2008(Latiffah et al, , 2009). However, the pathogenicity of F. solani on different orchids is not clear.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

A new ‘forma specialis’ of Fusarium solani causing leaf yellowing of Phalaenopsis

Chung¹,

Chen

Huang

et al. 2010

Plant Pathology

View full text Add to dashboard Cite

The pathogenicity of 35 Fusarium solani isolates obtained from diseased leaves of greenhouse-grown Phalaenopsis plants in Taiwan was tested on different orchids, including Phalaenopsis sp., Cymbidium spp., Oncidium sp., Dendrobium sp. and Cattleya sp., plus pea (Pisum sativum), chrysanthemum (Chrysanthemum indicum) and cucurbit [melon (Cucumis melo) and cucmber (C. sativus)] plants. Isolates of F. solani from Phalaenopsis spp. caused severe leaf yellowing on Phalaenopsis and mild symptoms on Cymbidium spp., but no visual symptoms on Oncidium sp., Dendrobium sp., Cattleya sp., pea, chrysanthemum or melon. Fusarium solani isolates collected from Phalaenopsis, pea and cucurbits were molecularly characterized by internal transcribed spacer (ITS), intergenic spacer (IGS) and b-tubulin gene analyses. Phylogenetic trees constructed by distance and parsimony methods indicated that isolates from Phalaenopsis were grouped into one type based on ITS, IGS and b-tubulin sequences with high bootstrap value (> 84%) support, compared to 'formae speciales' of F. solani from the other hosts. These analyses show that isolates of F. solani from Phalaenopsis are distinct from F. solani isolates from other hosts in Taiwan. Therefore, it is proposed that F. solani isolates that incite Phalaenopsis leaf yellowing be designated F. solani f. sp. phalaenopsis.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A new ‘forma specialis’ of Fusarium solani causing leaf yellowing of Phalaenopsis

Chung¹,

Chen

Huang

et al. 2010

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…could infect the Cymbidium and some isolates could infect Oncidium and Dendrobium plants (Srivastava et al., ). Previous studies revealed that F. proliferatum could cause diseases on Cymbidium (Ichikawa & Aoki, ), Phalaenopsis (Yang, ) and Dendrobium (Latiffah, Nur‐Hayati, Baharuddin, & Maziah, ; Swett & Uchida, ) but not on Oncidium . The F. proliferatum isolates from Cymbidium could infect and cause black spot symptoms on detached Oncidium leaves (with wounding) and seedlings (without wounding).…”

Section: Discussionmentioning

confidence: 99%

Identification of Fusarium proliferatum causing leaf spots on Cymbidium orchids in Taiwan

Wang

Chen

Jain

et al. 2018

Journal of Phytopathology

View full text Add to dashboard Cite

Previous research indicated that black and yellow leaf spots on Cymbidium, Ondontioda, Dendrobium and Cattleya could be caused by Fusarium proliferatum worldwide. However, the agent causing leaf spot on Cymbidium spp. plants is still obscure in Taiwan. Thirty‐five F. fujikuroi species complex (FFSC)‐like isolates were collected from Cymbidium leaf spot from different greenhouses in Taiwan. All isolates were identified as F. proliferatum based on morphological characteristics and molecular analysis. Sequence of translation elongation factor 1‐alpha gene showed 99%–100% homology with F. proliferatum. In addition, two assay techniques using either detached leaves or seedlings were used to evaluate the pathogenicity and host range of the isolates and consequently their effects on Cymbidium and other orchid plants. Pathogenicity assays revealed that all isolates induced black and necrotic spots on detached leaves of Cymbidium, showing 9.4%–29.5% severity on seedlings of Cymbidium. Results of host specificity tests on detached leaves of different plants indicated that the F. proliferatum isolates collected from Cymbidium plants caused severe black spots on Oncidium, Cymbidium, Dendrobium and Cattleya plants. The symptoms on Phalaenopsis plants were relatively mild. Results of host specificity tests on plant seedlings indicated that the F. proliferatum isolates of Cymbidium origin were also pathogenic to Oncidium, Cymbidium and Dendrobium, but not to Cattleya and Phalaenopsis. Phylogenetic analysis of the translation elongation factor (TEF) gene among all fungal isolates using maximum parsimony (MP) and Bayesian inference (BI) methods revealed that the isolates of F. proliferatum from Cymbidium spp. could be separated from other FFSC‐like species with high phylogenetic support.

show abstract

“…Two month-old grafted cashew seedlings were used for pathogenicity test. Pathogenicity test was performed on 9 cashew seedlings by spraying conidial suspensions (10 6 spores mL-1) of the isolates selected randomly on leaves of 9-month-old plants (Latiffah et al, 2009). Conidial suspensions were produced by growing mycelial mats of Cryptosporiopsis spp (AA1, AA2, AA3, AA4, AA5, AA6, AA7, AA8, AAA9 and AA10) on PDA in 9-cm Petri dishes for 10 days at 25°C in 12hr photoperiod and flooding with sterile distilled water containing a drop of Tween 80.…”

Section: Proving Pathogenicity Of Cryptosporiopsis Spp Causing Cashewmentioning

confidence: 99%

Biology and Infection Mechanisms of Cryptosporiopsis spp Fungus Causing Blight Disease on Cashew (Anacardium Occidentale L.)

Dominic¹,

Makobe²,

Agboton³

et al. 2014

JPS

View full text Add to dashboard Cite

Efficient isolation, purification and identification techniques of a new pathogenic fungus of cashew were developed.In the study, aspects related to identification, characterization of conidial morphology and infection of the Cryptosporiopsis spp fungus were investigated. Macroscopic observations of symptoms and signs of cashew blight disease as well as isolation, culturing and morphological characterization of its causative agent were carried out. Cryptosporiopsis spp fungus penetration and infection mechanisms on susceptible cashew leaves were studied. Cashew leaves were inoculated with fungal suspensions by pricking with pin, clipping with scissors and painting a fungal suspension onto the leaves with a paintbrush. Pathogenecity assays were performed on cashew leaves, nuts and apples. Irregular spot lesions, with a brown margin, formed on leaves especially on the young leaves. Direct conidial transfer was found to be the efficient method of isolation because it was less prone to contamination. Blight macroconidia are typical of Cryptosporiopsis having ellipsoidal, rounded at the apex, tapering into a scar at the base. Stomatal penetration of Cryptosporiopsis spp was visible through the simple staining technique using lactophenol solution. Penetration was by germinating conidia through a cuticle that caused an infection in leaves. Paint brush method of inoculation was found to be more effective as compared to other two methods based on the lesion size. Leaves inoculated with Cryptosporiopsis spp isolate began curling after 72 h thereafter developed dark, irregularly shaped spots with brown margins. An increased understanding of cashew blight is important for developing strategies for its management.

show abstract

Identification and Pathogenicity of Fusarium Species Associated with Root Rot and Stem Rot of Dendrobium

Cited by 27 publications

References 8 publications

A new ‘forma specialis’ of Fusarium solani causing leaf yellowing of Phalaenopsis

A new ‘forma specialis’ of Fusarium solani causing leaf yellowing of Phalaenopsis

Identification of Fusarium proliferatum causing leaf spots on Cymbidium orchids in Taiwan

Biology and Infection Mechanisms of Cryptosporiopsis spp Fungus Causing Blight Disease on Cashew (Anacardium Occidentale L.)

Contact Info

Product

Resources

About