Considering that the Minas frescal cheese is of great acceptance for the Brazilian consumers, it was considered necessary to monitor the quality of this food, as regards the physical-chemical aspects of moisture and fat and in relation to the presence of foreign matter. A total of 30 samples were analyzed of 5 different brands and 6 lots of each brand, purchased commercially from Grande ABC (SP) from April to September 2015. The moisture and fat tests were performed according to the methods described in the book Physicochemical methods for food analysis: analytical standards of the Adolfo Lutz Institute and the research of foreign matter according to the AOAC official methods. The results of the moisture analyzes revealed that 17% were in disagreement with the legislation by presenting values below the limit. As for the parameter fat in dry extract, 6% of analyzed samples were in disagreement with the current legislation by presenting values above the recommended procedure. Regarding the presence of foreign matter, at least 50% of samples from each brand had light filth and all the brands had presented fragments of rodent hair. The results demonstrate the lack of compliance with the parameters of product identity and quality, indicating the need for maintenance of good practices and constant surveillance of this food.
Food prepared with products derived from animals are involved in most cases of staphylococcal poisoning; therefore, the research of Staphylococcus spp. in Emmental cheese is more applicable. The objective of this study was to identify coagulase-negative Staphylococcus spp. (CNS) in cheese using biochemical and molecular techniques to detect the presence of nine genes responsible for the production of enterotoxins. From 180 samples analyzed, 204 CNS strains were obtained and identified as being 46 (22.6%) S. saprophyticus strains, 27 (13.2%) S. hominis spp. hominis strains, 22 (10.8%) S. sciuri strains, 21 (10.3%) S. xylosus strains, 19 (9.3%) S. epidermidis strains, 19 (9.3%) S. haemolyticus strains, 17 (8.3%) S. lentus strains, 17 (8.3%) S. warneri strains, 11 (5.4%) S. equorum strains and 5 (2.5%) S. cohnni . Using the PCRm protocol, 14 (6.9%) strains with the presence of the genes on the enterotoxin E (SEE)11 (78.6%), J (SEJ) 1 (7%), C (SEC) 1 (7%) and I (SEI) 1 (7%) were detected. Based on the results, the type of package is not interfered of growth and isolated that Staphylococcus spp. in cheese. It was observed that bacteria capacity to produce coagulase cannot be understood as an indicative of enterotoxigenicity; therefore, the CNS should be considered as a target of importance in the epidemiology of staphylococcal intoxications. It can be concluded that CNS need to be included in bacterial foodborne disease research, since the genes responsible for the production of toxins were detected and none of the studied samples presented Staphylococcus spp. counting above the limits allowed by legislation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.