Study of the transesterification of Calophyllum innophyllum seed oil from Kendari with methanol has been conducted. The purpose of the research to determine of the yield of ester produced in transesterification process with methanol, the viscosity of biodiesel produced, moisture content of biodiesel produced, density of biodiesel produced. The methods used in this study are sample preparation, transesterification process, biodiesel quality parameter test consisting of viscosity, moisture content and density. The results showed that good concentration of phosphoric acid was used to remove the gum is 85%, the yield of ester produced in the process of transesterification with methanol is 111,647%, parameter of biodiesel quality measured that viscosity = 0,315 mm2/ s not fulfill Indonesian National Standart (INS), water content is 0.02 meets the INS and the density = 0.8725 g / cm3 meets the INS standard

The kinetics of antibacterial activity of cajuput oil (Melaleuca cajuputi) to E. coli, B. cereus, and S. aureus were investigated. The aim of this study was to determine the reaction orders of cajuput oil as an antibacterial agent. The extraction of cajuput oil was conducted by water-steam distillation. The yield was 0.88%. GC-MS analysis showed that the cajuput oil contained β-ocimene (19.35%), 1.8-cineole (17.67%), limonene (12.09%), β-caryophyllene (9.51%), γ-terpinene (8.93%), 2-β-pinene (8.85%), α-terpinolene (4.96%), α-humulene (4.10%), α-terpineol (2.83%), and p-cymene (2.33%). The extract showed antibacterial activity to E. coli, B. cereus, and S. aureus, with the reaction orders of 0.4460, 0.8235 and 0.6928, respectively.

Kinetic studies effectiveness clove leaf (Syzigium aromaticum) oil as antifungal Candida albicans have been done. The study have purpose to determine the reaction order, reaction constants (k) and relationship the concentration of clove leaf (Syzigium aromaticum) oil every time (At) with the initial concentration of of clove (Syzigium aromaticum) oil (Ao) and time (t) and equipped determination of the minimum concentration of clove leaf (Syzigium aromaticum)oil effective anti-fungus Candida albicans. The results shows the anti-fungal activity clove leaf (Syzigium aromaticum) oil on Candida albicans for each variation of the concentration 100%, 75%, 50% and 25% are 14.2 mm, 12.2 mm, 10.8 mm and 10.4 mm respectively. Reaction order as antifungal of the clove leaf (Syzigium aromaticum) oil on Candida albicans is 0.2112 with k = 5.0594. The minimum concentration of clove leaf (Syzigium aromaticum) oil as anti-fungal Candida albicans is 17.86%.

Antibacterial activity test Staphylococcus aureus and Salmonella typhihas been performed by microencapsulation product of rogo essential oil (Premnaserratifolia Linn). This study aims to determine rogo oil activity test and microencapsulation results as antibacterial S. aureus and S. typhi. The results of antibacterial activity of rogo liquid oil to S. aureus and S. Typhi bacteria showed different inhibitory power of each concentration variation of 12.5%, 25%, 50% and 100% with 100% concentration as the best inhibitor for both bacteria. While the antibacterial activity test of S. aureus and S. typhi from microencapsulated rogo oil: maltodextrin showed the difference of each variation of concentration 1:10; 1:12; 1:14; 1:16 and 1:18 with a 1:14 composition of S. aureus and 1:18 in S. typhi as the best ratio of activity power. The antibacterial activity test between liquid rogo oil and microencapsulated result shows the difference, but it can be concluded that the inhibitory power of rogo oil from microencapsulation is more inhibited than 100% rogo oil.

This study aims to determine optimum time of action of the enzyme α-amylase and glucoamylase needed in hydrolyze of starch from waste processed cassava (Manihot esculenta Crantz var. Lahumbu). This research was conducted through three main stages, namely the gelatinization, liquefaction and saccharification. The method was used method are liquefaction and  saccharification. The variation time of the stage liquefaction: 12; 24; 36; 48; 60; and 72 minutes and the saccharification stage are: 9; 18; 27; 36; 45; 54; and 63 hours. The results showed that the optimum time required for stage liquefaction using α-amylase enzyme is 48 minutes on the condition of a temperature of 80 oC with a value of 0.09% amylose levels were measured using UV-Vis spectrophotometer. The optimum time required for saccharification step using a glucoamylase which is 54 hours on the conditions of a temperature of 50oC with the amount of reducing sugar concentration of 9.186 g/L as measured using a UV-Vis spectrophotometer.