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Silver nanoparticles synthesized from aqueous extract of mushroom Pleurotus ostreatus exhibited inhibitory effect at the concentration of 12.5, 25, 50, and 100 mg/ml against some pathogenic bacteria and fungi such as Candida albicans, Candida guilliermondii, Candida krusei, Candida zeylanoides, Geotrichum klebahnii, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The maximum inhibition zone was observed against C. zeylanoides at the concentration of 100 mg/ml was 24.5 mm, while the minimum inhibition zone was observed against Geotrichum at the concentration of 25 mg/ml was 8 mm and the concentration of 12.5 mg/ml was not effective against some species.

Nanotechnology involved tinkering to make important materials at atomic levels, manipulating and modifying substances 1, 00, 000 times smaller than a strand of human hair. It is expected that nanotechnology will revolutionize society as well as science. It involves one billionth of a meter of technology. The term (NANO) has been derived from the Greek word (Dwarf)[1].Nanotechnology is foreseen to significant influence science, economy and everyday life in twenty one century and it may become one of the driving forces to the next industrial revolution. Nanoparticles of Silver have many useful applications included spectrally selective coating for solar energy absorption and intercalation material for electrical batteries, as optical receptors

The study aims to biosynthesized of sliver nanoparticle from aqueous extract of olive leave and evaluate the effectiveness of the synthesis AgNPs against isolated fungi. The study mediating fifty samples were taken from various tools in laboratory from five hospitals in Baghdad. Four species of fungi were identified depending on the morphological and microscopic characteristics. The most common isolated fungi based on their frequency ratio were as follows Aspergillus niger 87.5%, Aspergillus flavus 62.5%, Aspergillus fumigatus 53.5% and Aspergillus nidulans 37.7%.The Biosynthesis of silver nanoparticle developed a rapid, eco-friendly and convenient green method for the stable silver nanoparticles (AgNPs) were synthesised with an average diameter of 30 ± 60 nm and like spherical in shape, using the aqueous solution of the Olive tree (Olea europaea) leaves extract.The reaction is carried out at 10-3M of silver nitrate. The AgNPs synthesized were confirmed by their change of color to (dark brown-grey). The characterization was studied using UV-Visible spectroscopy, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Inhibition effect of AgNPs against fungi has been studied using well diffusion method by studying  the effect of different concentration (100, 75, 50 and 25). The results revealed that the AgNPs have considerable antifungal activity comparison with alcohol. The obtained results indicate that the highest level of inhibition zone was detected at the concentration of 100 µg/ml of AgNPs, where the inhibition zones are (23.33 ± 4.41) for A. flavus and the lowest level of  inhibition zone was detected at  the concentration 25 µg/ml of AgNPs ,where the inhibition zones are (6.00 ± 1.15) for A.nidalus. While using alcohol the highest level of inhibition zone was detected at the concentration of 100 µg/ml of Alcohol, where the inhibition zones are (12.33 ± 1.45) for A.nidalus, and the lowest level of  inhibition zone was detected at  the concentration 25 µg/ml of Alcohol ,where the inhibition zones are (4.67± 0.33) for A.flavus.