Investigating the Inhibitory Effect of Silver Nanoparticles against Some Species of Candida and Pathogenic Bacteria

Abstract

Silver nanoparticles synthesized from aqueous extract of mushroom Pleurotus ostreatus exhibited inhibitory effect at the concentration of 12.5, 25, 50, and 100 mg/ml against some pathogenic bacteria and fungi such as Candida albicans, Candida guilliermondii, Candida krusei, Candida zeylanoides, Geotrichum klebahnii, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The maximum inhibition zone was observed against C. zeylanoides at the concentration of 100 mg/ml was 24.5 mm, while the minimum inhibition zone was observed against Geotrichum at the concentration of 25 mg/ml was 8 mm and the concentration of 12.5 mg/ml was not effective against some species.

Detection of Anti-cancer Activity of Silver Nanoparticles Synthesized using Aqueous Mushroom Extract of Pleurotus ostreatus on MCF-7 Human Breast Cancer Cell Line

“…1 filter paper. Finally, the collected aqueous extract was dried using a dryer freeze and the resulting powder was stored at 4 ± 2°C until used [18][19].…”

“…The stock solution of silver nitrate (AgNO3) was prepared by adding 1×10 -3 mg of silver nitrate powder to sterile deionized water D.W, and then the succeeding concentrations of 12.5, 25, 50, 100 and 200 µg /mL were made from it [19]. The volume 10 mg/ml of aqueous extract solution was prepared using D.W and then it was passed through a syringe-filter (0.2 µm).…”