Background: Histopathological diagnosis remains the gold standard for the diagnosis of cancer, including colorectal cancer, but it is infeasible when tumor tissue is not available. With the recognition of long non-coding RNAs (lncRNAs), the expression of lncRNAs in serum or tissue samples has been reported as a diagnosis method for some cancers, however, the diagnostic value of lncRNAs for colorectal cancer remains unclear. Methods: A systematic review and meta-analysis were conducted. Eligible studies were identified through a comprehensive literature search in PubMed, PubMed Central, Web of Science, Embase, and Cochrane Library (up to May 05, 2020) according to the selection criteria. Meta-DiSc, Review Manager and STATA were used to analyze the association between lncRNAs expression and the diagnosis of colorectal cancer. Results: Fifteen studies that analyzed the expression of 15 lncRNAs in 1434 CRC patients were included. The summary area under the curve (AUC) of lncRNA for the diagnosis efficacy between patients with and without CRC was estimated to be 0.8629, corresponding to a weighted sensitivity of 0.75 (95% CI: 0.72-0.77), specificity of 0.80 (95%CI: 0.78-0.82). Subgroup analysis illustrated that the AUC of blood-based detection of lncRNA showed 0.8820, pooled DOR: 18.57, while tissue-based analysis showed 0.8203, pooled DOR: 10.47. Blood-based tests were then divided into two categories, plasma-based and serum-based lncRNA testing. Results revealed that the AUC of serum-based detection was 0.9077, pooled DOR: 26.64, and plasma-based detection was 0.5000, pooled DOR: 11.80. Conclusions: This meta-analysis indicates that the aberrantly expressed lncRNAs might serve as potential diagnostic biomarkers for CRC patients and blood-based lncRNA analysis is of higher diagnostic accuracy than tissue-based testing. Moreover, serum-based lncRNA testing achieved higher diagnostic efficacy than plasma-based analysis.
Steam distillation oil (SDO) and simultaneous distillation oil (SDEO) were obtained from Tagetes Erecta L. leaf of Heilongjiang province by steam distillation method and simultaneous distillation method, respectively. The yields were 0.0943% and 1.005%. Compared the differentiation of essential oil through the GC-MS analysis, the result indicated that there were twenty-nine kinds of compounds can be confirmed from SDO and fifty-one kinds can be confirmed from SDEO. The two kinds of essential oil were assayed for affinity to scavenge sodium nitrite for the purpose of characterizing mechanisms of nitrite cleaning activity. Both show strong nitrite cleaning activity up to a concentration of 0.1mL.The maximum scavenging rate was 95.18% (SDO) and 54.28% (SDEO).
Due to an enhancement effect of Lumino-KBrO3 chemiluminescence system induced by nitrite, nitrite in sauerkraut was determined using chemiluminescence method. The effects of reaction substance concentrations, acidity, and interfering ions on chemiluminescence system were investigated to explore the optimal conditions for nitrite determination. Results revealed that the optimal reaction conditions of chemiluminescence were 0.05MH2SO4, 0.06MKBrO3, 1.50×10-4MLuminol and 0.25MNaOH in the chemiluminescence system. The detection limit (S/N=3)for NO2-was 8×10-11g/mL and an excellent linear relationship was exhibited in the range of 0.001-10mg/L. Eleven replicate determinations for nitrite in sauerkraut at 1.00mg/kg exhibited an average recovery rate of 101.00% and a relative standard deviation of 2.90%. Therefore, it revealed that the method not only possessed high sensitive and selective characteristics, but also possessed rapid, simple and convenient characteristics, compared with traditional methods.
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