The detailed kinetic parameters indicated that COMT provide convenience for the next glucuronidation; monkey would be a preferred animal model for the preclinical investigation of baicalein. Importantly, oroxylin A should be reconsidered in evaluating baicalein efficacy against inflammatory diseases.
Background: Dysregulation of micro-RNAs (miRNAs) is profoundly linked to inflammatory bowel diseases (IBD), but little is known about the specific biological functions of miRNAs in IBD. This study sought to elucidate the effect and the underlying target of miR-29c-3p in ulcerative colitis (UC). Methods: The levels of miR-29c-3p and leukemia inhibitory factor (LIF) were measured in inflamed lesions of UC patients and dextran sulfate sodium (DSS)-induced colitis mice by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. MiR-29c-3p was predicted to target LIF by bioinformatics software, which was verified via luciferase reporter assay and transfection of miR-29c-3p mimics or inhibitor. The role of miR-29c-3p/ LIF axis in intestinal inflammation was explored in experimental colitis mice and Caco-2 cells. Results: MiR-29c-3p was markedly downregulated while LIF was upregulated in colon tissues of UC patients and DSS-challenged colitis mice as well as in primary intestinal epithelial cells (IECs) and LPS-treated Caco-2 cells. MiR-29c-3p inhibited LIF expression at the transcriptional level via binding to LIF 3ʹ-untranslated region (UTR) in Caco-2 cells. Targeting miR-29c-3p/LIF axis regulated inflammatory cytokines production, cell proliferation and apoptosis. Overexpression of miR-29c-3p aggravated mice experimental colitis via suppressing LIF.
Conclusion:Our findings demonstrate that the upregulation of miR-29c-3p promotes gut inflammation and the expression of pro-inflammatory mediators via suppressing LIF, thereby modulating the pathogenesis of UC.
Coal resources in China are developed in several regions with shallow groundwater, and large mining-related surface subsidence can have negative impacts on agriculture, land and water resources as well as existing and future socio-economic resources. All these are important for sustainable resource development. Dynamic subsidence reclamation (DSR) planning concepts are evaluated here for another case study with analyses over a 11-year period. In DSR topsoil, subsoil, farming, and water resources management are dynamically synergized concurrent with mining ahead of and behind the projected dynamic subsidence trough. The study area involved mining five longwall faces (and post-mining reclamation) to assess if DSR could have improved both the environment and socio-economic conditions for post-mining land use as compared to using traditional reclamation (TR) and TR-modified (TR(MOD)) approaches. The results show that: (1) Upon final reclamation, farmland area and water resources in DSR and TR (MOD) will have increased by 5.6% and 30.2% as compared to TR. Removing soils ahead of mining before they submerge into water is important for farmland reclamation and long-term economic development. (2) Due to topsoil and subsoil separation and storage in the DSR plan, reclaimed farmland productivity should recover quickly and agriculture production should be larger than TR and TR(MOD) plans. (3) For a simplified economic model, the total revenue in the DSR plan should be 2.8 times more than in TR and 1.2 times larger than in TR (MOD) plan. (4) The total net revenue of the TR(MOD) plan should be increased by 8.1% as compared with the TR plan. The benefits will be much greater for analyses over longer periods. Overall, the DSR plan will allow for an improved socio-economic environment for new businesses to support disrupted workforces during and after mining.
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