Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction. It is, therefore, critical to develop a point-of-care testing technology for myoglobin detection. In this work, we reported a sensitive plasmonic immunoassay-based on enzyme-mediated localized surface plasmon resonance change of gold nanorods for the point-of-care testing detection of myoglobin. In addition, we developed a novel plasmonic immunoassay reader using the ambient light sensor of smart phone to increase the accessibility and utility of the plasmonic immunoassay. The linear detection range of gold nanorods-based plasmonic immunoassay for myoglobin detection was 0.1–1000 ng mL−1 and the limit of detection was 0.057 ng mL−1. Myoglobin in serum samples was also analyzed by the plasmonic immunoassay. The results were significantly correlated with those of conventional enzyme-linked immunosorbent assay. The plasmonic immunoassay, coupled with smart phone-based reader, could be widely used for point-of-care testing application of acute myocardial infarction, especially in the regions with limited technological resources.Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2806-9) contains supplementary material, which is available to authorized users.
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