Association analysis shown that the level of sICAM-1, VCAM-1, MCP-1 and vWF elevated in CHD with T2DM patients. Vascular endothelial dysfunction could be caused to the coronary artery stenosis pathophysiological process. Results from this study suggested that sICAM-1, VCAM-1, MCP-1 and vWF may contribute to the occurrence and development of vascular lesions in T2DM. These endothelial function related factors could be acceptable as a prediction and testing index of vascular complications in T2DM.
BackgroundCedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line.MethodsWe extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry.ResultsThe CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC50 values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells.ConclusionAt low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC50 values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis.
One of the most common complications associated with diabetes mellitus (DM) is diabetic peripheral neuropathy (DPN) (Dixit & Maiya, 2014), which might cause the formation of foot ulcers or even amputations (Tesfaye et al., 2013). Approximately 30% of patients with DPN experience neurological pain (Jacovides et al., 2014).Pain caused by DPN manifests as allodynia (pain caused by innocuous stimuli), hyperalgesia, spontaneous pain and a small amount of sensory deficit (Tesfaye et al., 2013). It usually starts in the distal toe, which is controlled by the peripheral axon, shows bilateral symmetry, and gradually progress to the proximal limb. Based on some studies, fingers and forearm might be affected as well (Tesfaye et al., 2011(Tesfaye et al., , 2013. DPN-related pain has a complex mechanism of pathogenesis. Dorsal root ganglion (DRG) is the hub that connects peripheral and central pain signals; abnormal DRG neurons can directly induce pain (Hoeijmakers et al., 2014;Schreiber et al., 2015). Symptomatic treatment is used to relieve pain associated with DPN. Pain relievers, such as anticonvulsants (pregabalin), antidepressants (duloxetine) and opioids have limited efficacy in treating the pain associated with DPN (Schreiber et al., 2015). Therefore, it is
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.