Sargentodoxa cuneata decoction has been used to treat arthritis in China for hundreds of years. Herein, the polysaccharide fraction (PSC) purified from S. cuneata was evaluated for its in vitro and in vivo anti-inflammatory effects. PSC and its sub-fractions PSCA-1 and PSCB-1 significantly suppressed nitric oxide (NO) release in LPS-induced RAW264.7 cells by down regulating the inducible nitric oxide synthase (iNOS) level. Furthermore, PSC markedly inhibited carrageenan induced rat hind paw edema, decreased in hind paw, serum and liver malondialdehyde (MDA) levels and prostaglandin E (PGE ) levels. In addition, PSC increased superoxide dismutase (SOD) activity in serum and liver of the rats. These results revealed that the polysaccharide obtained from S. cuneata (PSC) possessed potent anti-inflammatory activity and may be one of the important bioactive constituents from the plant responsible for the anti-arthritis effect.
Sargentodoxa cuneata is widely used in the treatment of rheumatic arthritis in China. This study was conducted to investigate the anti-inflammatory and immunoregulatory activities of the ethanol extracts from S. cuneata and analyze the bioactive constituents. The ethyl acetate (ESCe), dichloromethane (ESCd) and aqueous (ESCa) fractions at a dose of 200 mg/kg significantly inhibited mice ear edema. Furthermore, the ethyl acetate (ESCe) and aqueous fractions (ESCa) inhibited rat paw edema, decreased the levels of Malondialdehyde (MDA) and biosynthesis of the prostaglandin (PGE 2), increased superoxide dismutase (SOD) activity, inhibited adjuvant arthritis (AA) rat paw edema, enhanced splenocyte proliferation and macrophage phagocytosis activity. ESCa at a dose of 50 mg/kg significantly suppressed IL-1β and TNF-α levels to 0.101 ng and 15.45 pg in each million macrophages. S. cuneata possessed anti-inflammatory and immunoregulatory activities, and phenolic compounds are the important bioactive constituents of this plant.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.