This study is an attempt to generate genetic database for three endogamous populations of Sikh population (Arora, Jat and Ramgariha) of Central India. The analysis of eight autosomal STR loci (D16S539, D7S820, D13S317, FGA, CSF1PO, D21S11, D18S51, and D2S1338) was done in 140 unrelated Sikh individuals. In all the three studied populations, all loci were in Hardy -Weinberg equilibrium except at locus FGA in Ramgariha Sikh and locus D16S539 in Arora Sikh. An analysis of molecular variance (AMOVA) showed 1% variation among the three studied populations. The close genetic relationship between Jat and Ramgariha Sikh population were confirmed in the MDS Plot generated from the pairwise genetic distances.
The present biochemical study is planned primarily to characterize genetically the Gond Tribe of Madhya Pradesh. The blood samples for the present study were collected at random from a total of 200 apparently healthy and not closely related individuals of either sex, of the Gond of Hoshangabad, Betul and Sehore districts of Madhya Pradesh. The samples were analyzed for phenotypes of A1A2BO and Rh (D) blood groups by standard tube method and for Red Cell Enzymes electrophoretic method.Typed Red Cell Enzymes were Adenosine Deaminase, Acid Phosphatase locus 1, Phosphoglucomutase locus 1, Esterase D, Adenylate kinase locus 1 and Glucosephosphate isomerase. A rare allele ACP*C was recorded in Gond tribe despite the fact that it was totally absent not only in Keer of Betul, but also in Korku of Pachmarhi Hoshangabad and Bhils of Jhabua. In case of GPI, rare variant (GPI*1-7) was recorded in Gond Tribe of Hoshangabad of the State. There was great heterogeneity (h) values over the loci in the Gond material, varying from as low as 0.0304 at GPI locus to as high as 0.6244 at A1A2BO locus. Analysis of heterozygosity revealed that in the Gond tribe GPI was the least variable locus and A1A2BO was the most variable locus. Genetic relationships among the present Gond tribe and earlier studied Tribal and Caste Populations of Neighboring States of Gujarat and Rajasthan shows that the Hindu and Muslim separated out from the Tribal population of neighboring States from earlier stage of evolution. Rajasthan Bhil shows distant single line subcluster, the latter tribes were placed together in an another subcluster. In addition all the Tribes of Gujarat and Madhya Pradesh shows close genetic affi nities.
Hemoglobinopathies are associated with the inherited diseases characterized by structural or biochemical defects in haemoglobin chain. HBB (Human β-globin) gene relates naturally occurring genetic variation in humans. The purpose of the study is to analyze exon specic HBB gene mutations and to observe diversity in all 3 exons of HBB gene by Single Nucleotide Polymorphism (SNP) marker, among the three communities of M.P. ie. Raj-Gond, Baiga Kunbi. Total 520 samples are amplied with exon specic primers of HBB gene, followed by Polymerase Chain Reaction (PCR). Sequence analysis of 97.71% good quality sequences was performed followed by SNP-BLAST online tool and then analyzed for notied SNP against database at NCBI. Molecular marker based genetic diversity was observed and phylo-genetically analyzed by dendogram. Total 191 sites in HBB1 and HBB2 region were detected as SNP variant and no polymorphic sites were observed in HBB3 region. The SNP variant site detected in the study are 3383 (T→C, rs: 63750898), 3466 (G→C, rs: 33960103) in HBB1 region, 3835 (G→C, rs: 63750898), 3893 (T→G, rs: 7480526) and 3900 (C→T, rs: 7946748) in HBB2 region. The BLAST-X analysis identied the presence of CDS from average ~ 127 positions. The SNP study was followed by phylogentic analysis for genetic diversity/distance/variation. Protein structure study can be carried out which allows visualization of the locations of mutation on the 3D structure.
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