Background/Aims Proprioception acuity is important in sports activities and stretching is widely used in warm-up programmes. The main objective of this study was to evaluate if a warm-up programme with and without stretching flexors and extensors muscles could affect knee joint position sense. Methods The effects of different stretching regimens on joint position sense were examined. A total of 12 semi-professional football players completed four warm-up sessions over 4 weeks: standard warm-up programme; standard warm-up programme with quadriceps stretching; standard warm-up programme with hamstring stretching; and standard warm-up programme with stretching of both quadriceps and hamstrings. Open kinetic chain knee joint position sense was estimated from the ability to reproduce the three target angles (20°, 45° and 60° knee flexion) in the dominant limb before and after the intervention. Results In the absolute angular error, there was a statistically significant three-way interaction between the warm-up programme, target angle and time (F (6, 54)=6.88, P=0.001). Findings of post-hoc analysis demonstrated that there was a statistically significant difference between the pre- and post-stretching of hamstrings for the target angles of 20° (4.70 vs 1.57, P=0.01), 45° (1.70 vs 4.50, P=0.02), and 60° (1.93 vs 4.20, P=0.02). In the relative angular error, interaction of time by the warm-up programme was significant (F (3, 27)=3.41, P=0.03). Conclusions The warm-up programme with static stretching of hamstrings had a negative effect on open kinetic chain knee joint position sense during the flexion to extension repositioning task, which may not only have a negative effect on performance of athletes as a part of warm-up exercises, but may also lead to further injuries.
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