In recent years, there has been an increase in pesticide use to improve crop production due to the growth of agricultural activities. Consequently, various pesticides have been present in the environment for an extended period of time. This review presents a general description of recent advances in the development of methods for the quantification of pesticides used in agricultural activities. Current advances focus on improving sensitivity and selectivity through the use of nanomaterials in both sensor assemblies and new biosensors. In this study, we summarize the electrochemical, optical, nano-colorimetric, piezoelectric, chemo-luminescent and fluorescent techniques related to the determination of agricultural pesticides. A brief description of each method and its applications, detection limit, purpose—which is to efficiently determine pesticides—cost and precision are considered. The main crops that are assessed in this study are bananas, although other fruits and vegetables contaminated with pesticides are also mentioned. While many studies have assessed biosensors for the determination of pesticides, the research in this area needs to be expanded to allow for a balance between agricultural activities and environmental protection.
Mancozeb is a worldwide fungicide used on a large scale in agriculture. The active component and its main metabolite, ethylene thiourea, has been related to health issues. Robust, fast, and reliable methodologies to quantify its presence in water are of great importance for environmental and health reasons. The electrochemical evaluation of mancozeb using a low-cost electrochemical electrode modified with poly (3,4-ethylene dioxythiophene), multi-walled carbon nanotubes, and gold nanoparticles is a novel strategy to provide an in-situ response for water pollution from agriculture. Additionally, the thermal-, electrochemical-, and photo-degradation of mancozeb and the production of ethylene thiourea under controlled conditions were evaluated in this research. The mancozeb solutions were characterized by electrochemical oxidation and ultraviolet-visible spectrophotometry, and the ethylene thiourea concentration was measured using ultra-high-performance liquid chromatography high-resolution mass spectrometry. The degradation study of mancozeb may provide routes for treatment in wastewater treatment plants. Therefore, a low-cost electrochemical electrode was fabricated to detect mancozeb in water with a robust electrochemical response in the linear range as well as a quick response at a reduced volume. Hence, our novel modified electrode provides a potential technique to be used in environmental monitoring for pesticide detection.
Organic conductive polymers have been widely used as active layers in bioelectronic devices. In this work, a novel approach to entrap enzymes directly into the conductive active layer is described, using a polysaccharide as a surfactant. The surfactant allowed the electropolymerization from a micellar media and it acted as a doping agent in the conductive polymer. Gold nanotriangles were added to the matrix in order to enhance the enzymatic product quantification. The composition and oxidation state of the biocompatible conductive layer were confirmed by infrared spectrophotometric and Raman studies. Meanwhile, the gold nanotriangles presence, distribution and electrochemical activity were studied by transmission electron microscopy, atomic force microscopy, dynamic light scattering and cyclic voltammetry techniques. The inhibition of the enzyme, due to the presence of pesticides, was used to electrochemically quantify their concentration in real water samples. The concentration was confirmed by gas and liquid chromatography. Therefore, this novel composite active layer allows building a biosensor with suitable performance for an early warning in environmental control, especially in countries highly impacted by agricultural activities.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.