Raster image correlation spectroscopy is a powerful tool to study fast molecular dynamics such as protein diffusion or receptor-ligand interactions inside living cells and tissues. By analysing spatio-temporal correlations of fluorescence intensity fluctuations from rasterscanned microscopy images, molecular motions can be revealed in a spatially resolved manner. Because of the diffraction-limited optical resolution, however, conventional raster image correlation spectroscopy can only distinguish larger regions of interest and requires low fluorophore concentrations in the nanomolar range. Here, to overcome these limitations, we combine raster image correlation spectroscopy with stimulated emission depletion microscopy. With imaging experiments on model membranes and live cells, we show that stimulated emission depletion-raster image correlation spectroscopy offers an enhanced multiplexing capability because of the enhanced spatial resolution as well as access to 10-100 times higher fluorophore concentrations.
ABSTRACT:Due to ever more efficient and accurate laser scanning technologies, the analysis of 3D point clouds has become an important task in modern photogrammetry and remote sensing. To exploit the full potential of such data for structural analysis and object detection, reliable geometric features are of crucial importance. Since multiscale approaches have proved very successful for image-based applications, efforts are currently made to apply similar approaches on 3D point clouds. In this paper we analyse common geometric covariance features, pinpointing some severe limitations regarding their performance on varying scales. Instead, we propose a different feature type based on shape distributions known from object recognition. These novel features show a very reliable performance on a wide scale range and their results in classification outnumber covariance features in all tested cases.
ABSTRACT:In this paper, we present a novel framework for the semantic labeling of airborne laser scanning data on a per-point basis. Our framework uses collections of spherical and cylindrical neighborhoods for deriving a multi-scale representation for each point of the point cloud. Additionally, spatial bins are used to approximate the topography of the considered scene and thus obtain normalized heights. As the derived features are related with different units and a different range of values, they are first normalized and then provided as input to a standard Random Forest classifier. To demonstrate the performance of our framework, we present the results achieved on two commonly used benchmark datasets, namely the Vaihingen Dataset and the GML Dataset A, and we compare the results to the ones presented in related investigations. The derived results clearly reveal that our framework excells in classifying the different classes in terms of pointwise classification and thus also represents a significant achievement for a subsequent spatial regularization.
ABSTRACT:In this paper, we address the classification of airborne laser scanning data. We present a novel methodology relying on the use of complementary types of geometric features extracted from multiple local neighbourhoods of different scale and type. To demonstrate the performance of our methodology, we present results of a detailed evaluation on a standard benchmark dataset and we show that the consideration of multi-scale, multi-type neighbourhoods as the basis for feature extraction leads to improved classification results in comparison to single-scale neighbourhoods as well as in comparison to multi-scale neighbourhoods of the same type.
ABSTRACT:The semantic labeling of 3D point clouds acquired via airborne laser scanning typically relies on the use of geometric features. In this paper, we present a framework considering complementary types of geometric features extracted from multi-scale, multi-type neighborhoods to describe (i) the local 3D structure for neighborhoods of different scale and type and (ii) how the local 3D structure behaves across different scales and across different neighborhood types. The derived features are provided as input for several classifiers with different learning principles in order to show the potential and limitations of the proposed geometric features with respect to the classification task. To allow a comparison of the performance of our framework to the performance of existing and future approaches, we evaluate our framework on the publicly available dataset provided for the ISPRS benchmark on 3D semantic labeling.
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