Globally, diarrhoea is the second commonest infectious cause of death in children less than 5 years old. It is estimated that more than one billion diarrhoea episodes occur every year causing up to 700,000 deaths among children younger than 5 years of age. Seventy-two percent of these deaths occur in children below two years and enteric viruses have been recognized as a major cause of childhood diarrhoea. This study was undertaken to determine the prevalence of enteric Adenoviruses and Rotaviruses in children with diarrhoea in rural Enugu communities of Enugu State South East Nigeria. Methods: Stool samples were collected from children less than 5 years with diarrhoea seen in any of the participating hospitals in Enugu State. Samples were collected between June 2015 and May 2017. Detection of rotavirus and enteric adenovirus antigens were performed using commercially available ELISA kit (Oxoid-ProspecT®). Demographic data of the children were also collected. Results: Of the 290 stool samples that had sufficient materials for adenovirus and rotavirus ELISA, 14 (4.8%) and 89 (30.7%) were positive for enteric adenovirus and rotavirus respectively. 3 (1%) were co-infected with adenovirus and rotavirus. Rotavirus positive cases were more among hospitalized patients while enteric adenovirus was more among outpatients. Marked peaks of rotavirus positivity were seen in January of each year but no peak was seen among adenovirus positive cases. Higher vomiting frequencies and severe dehydration were more among rotavirus positive cases compared to adenovirus positive cases (p = 0.030 and 0.001 respectively). Conclusion: Many diarrhoea cases among children aged <5 in the population studied were associated with enteric adenoviruses and rotavirus. This finding suggests that en
In Nigeria, dry season garden eggs are often associated with higher fungal contamination than rain-fed garden eggs and most reports on the identified pathogens were on post-harvest crops. There is possibility that some of the implicated pathogens can be traced to the farm. Thus, the present study aimed to identify the predominant fungi contaminants of dry season pre-harvest garden egg for possible bio-control measures. Sampled pre-harvest garden eggs grouped into symptomatic and asymptomatic types were collected from three farms within January to March for two consecutive years. Standard phenotypic identification methods were adopted. The most predominant isolates were further subjected to ITS rRNA sequencing. The applied methods identified 7 common isolates across the farms in the ascending frequency of Fusarium thapsinum (6.67±10.34%) <Colletotrichum spp (11.10±17.20%)<Fusarium oxysporum (14.43±22.36%) <Fusarium solani (23.70±20.32%) <Penicillium citrinum (35.57±9.12%) <Pichia spp (52.20±7.52%)< Aspergillius niger (66.67±5.99%) which were all significant (P<0.05). Among the isolates, a higher variance of 44.5 was observed within the three farms than within their months of recovery (14.8 variance). The fresh asymptomatic garden egg had 71.4% contaminants as against 100% from symptomatic ones. Molecular assay confirmed the most predominant isolates as Aspergillus niger 41 and Pichia kudriavzeviiC101 with assigned NCBI accession numbers of ON417062 and ON417063 after their sequence deposition in Genbank. High percentage recovery of these isolates especially from asymptomatic garden eggs gave an insight of possible health problems associated with it on consumption and this calls for appropriate bio-control measures from the farm
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