The caves have a significant economic importance in their role as tourist attraction and are of great importance for the presence of some rare species. The fauna identification in the "Grotta del Maresciallo" cave, situated in the Riviera di Ulisse Regional Park, started in march 2013 by a visual census and molecular approach. The studies allowed to detect 12 classes, 46 families, 47 species with their ecological niches, and the percentage contribution of each group to the Mediterranean marine cave diversity. Furthermore, we report the presence of two thermophilic species, as the star coral, Astroides calycularis (Pallas, 1766) and the goldblotch grouper, Epinephelus costae (Steindachner, 1878); the tropical species, the ringneck blenny Parablennius pilicornis (Cuvier, 1829); the endangered species, the dusky grouper Epinephelus marginatus (Lowe, 1834) included in the IUCN Red List; the rare species, the black brotula Grammonus ater (Risso, 1810), and some uncommon species such as the golden coral shrimp, Stenopus spinosus (Risso, 1826) and the spotted bumblebee shrimp, Gnatophillum elegans (Risso, 1816). Species of economic and medical interest were also recorded. In a DNA barcoding approach, Neighbour Joining (NJ) phylogenetic tree of 25 mitochondrial cytochrome oxidase subunit I species sequences, indicates that COI gene is suitable for an unambiguous identification. This first geological and biological attempt at the Marine Area of the "Grotta del Maresciallo" provides useful indications to focus future investigations, and may become a potential management tool for local administrations to protect these habitats.
The species identification is a key problem throughout the life cycle of fishes: from eggs and larvae to adults in ecosystem and fisheries research and control, as well as processed fish products labelling in consumer protection. Here, we report a rapid blue biotechnological method applied for egg and fish species discrimination. The amplification of a region of the mitochondrial genome, the cytochrome b, by using the Polymerase Chain Reaction (PCR) permits marine aquatic species identification. The obtained PCR-products were cut with different restriction endonucleases resulting in species-specific Restriction Fragment Length Polymorphisms (RFLP), which allowed to discriminate, in one step, between different types of caviar substitute species. The following analysis of mitochondrial DNA to control the labelling between caviar substitute species of Cyclopterus lumpus, Mallotus villosus, Trisopterus minutus minutus and the caviar, Acipenser baerii and their monitoring and surveillance is suitable with only one restriction enzyme, MboII. This blue method, applied to a very significant number of samples, retrieved online, allowed a rapid and economic identification of the species, with high percentage of correct identification for fish caviar substitute.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.