The weak thermal polarization of nuclear spins limits the sensitivity of MRI, even for MR-sensitive nuclei as fluorine-19. Therefore,d espite being the source of inspiration for the development of background-free MRI for various applications,including for multiplexed imaging,the inability to map very low concentrations of targets using 19 F-MRI raises the need to further enhance this platformsc apabilities.H ere, we employt he principles of CEST-MRI in 19 F-MRI to obtain a900-fold signal amplification of abiocompatible fluorinated agent, which can be presented in a" multicolor" fashion. Capitalizing on the dynamic interactions in host-guest supramolecular assemblies in an approach termed GEST,w e demonstrate that an inhalable fluorinated anesthetic can be used as as ingle 19 F-probe for the concurrent detection of micromolar levels of two targets,w ith potential in vivo translatability.F urther extending GEST with new designs could expand the applicability of 19 F-MRI to the mapping of targets that have so-far remained non-detectable.
The accumulated knowledge regarding molecular architectures is based on established, reliable, and accessible analytical tools that provide robust structural and functional information on assemblies. However, both the dynamicity and low population of noncovalently interacting moieties within studied molecular systems limit the efficiency and accuracy of traditional methods. Herein, the use of a saturation transfer‐based NMR approach to study the dynamic binding characteristics of an anion to a series of synthetic receptors derived from bambusuril macrocycles is demonstrated. The exchange rates of BF4− are mediated by the side chains on the receptor (100 s−1<kex<5000 s−1), which play a critical role in receptor‐anion binding dynamics. The signal amplification obtained with this approach allows for the identification of different types of intermolecular interactions between the receptor and the anion, something that could not have been detected by techniques hitherto used to study molecular assemblies. These findings, which are supported by a computational molecular dynamic study, demonstrate the uniqueness and added value of this NMR method.
The weak thermal polarization of nuclear spins limits the sensitivity of MRI, even for MR-sensitive nuclei as fluorine-19. Therefore,d espite being the source of inspiration for the development of background-free MRI for various applications,including for multiplexed imaging,the inability to map very low concentrations of targets using 19 F-MRI raises the need to further enhance this platformsc apabilities.H ere, we employt he principles of CEST-MRI in 19 F-MRI to obtain a900-fold signal amplification of abiocompatible fluorinated agent, which can be presented in a" multicolor" fashion. Capitalizing on the dynamic interactions in host-guest supramolecular assemblies in an approach termed GEST,w e demonstrate that an inhalable fluorinated anesthetic can be used as as ingle 19 F-probe for the concurrent detection of micromolar levels of two targets,w ith potential in vivo translatability.F urther extending GEST with new designs could expand the applicability of 19 F-MRI to the mapping of targets that have so-far remained non-detectable.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.