Single Fluorinated Agent for Multiplexed ¹⁹F‐MRI with Micromolar Detectability Based on Dynamic Exchange

Abstract

The weak thermal polarization of nuclear spins limits the sensitivity of MRI, even for MR-sensitive nuclei as fluorine-19. Therefore,d espite being the source of inspiration for the development of background-free MRI for various applications,including for multiplexed imaging,the inability to map very low concentrations of targets using 19 F-MRI raises the need to further enhance this platformsc apabilities.H ere, we employt he principles of CEST-MRI in 19 F-MRI to obtain a900-fold signal amplification of abiocompatible fluorinated agent, which can be presented in a" multicolor" fashion. Capitalizing on the dynamic interactions in host-guest supramolecular assemblies in an approach termed GEST,w e demonstrate that an inhalable fluorinated anesthetic can be used as as ingle 19 F-probe for the concurrent detection of micromolar levels of two targets,w ith potential in vivo translatability.F urther extending GEST with new designs could expand the applicability of 19 F-MRI to the mapping of targets that have so-far remained non-detectable.

Recent Research Progress of 19F Magnetic Resonance Imaging Probes: Principle, Design, and Their Application

“…obtained a biocompatible small molecular fluorinated agent with 900‐fold signal amplification, which could be presented in a “multicolor” fashion, as shown in Figure 2c. [ 39 ]…”