Codonopsis pilosula (CP) is a traditional Chinese medicine used to invigorate spleen, replenish lung, nourish blood and engender fluid. A rapid, selective and sensitive ultra-performance LC-tandem mass spectrometry method was developed and validated to determine lobetyolin in rat plasma. The calibration curve showed good linearity over a concentration range of 0.46-1000 ng/mL for lobetyolin. The extraction recovery ranged from 72.5% to 89.1% with matrix effects of 81.6%-107.8%.The intra-and inter-batch precision and accuracy were 0.02-14.4% and −13.9% to −1.36%, respectively. The method was successfully applied for the bioavailability study of lobetyolin in rats after oral administration of pure lobetyolin and CP extract.Results showed that the elimination half-time (t 1/2 ) and the area under the concentration-time curve from zero to infinity of lobetyolin in CP extract were statistically different from those of the pure monomer (P < 0.05). However, the time to reach the maximum plasma concentration (T max ) and the maximum concentration (C max ) showed no significant differences between the two treatments. Furthermore, the bioavailability of lobetyolin in the experimental group was only 3.90%, significantly lower than that of the CP extract group (6.97%). The low bioavailability indicated that this component may be absorbed poorly or metabolized extensively in rats. Our results will provide useful information for further preclinical studies and formulation preparation of lobetyolin.
Aucklandia lappa Decne. has been used as a traditional Chinese herb for thousands of years in treating various kinds of disorders. According to the Chinese Pharmacopoeia, there are two kinds of processed products, raw and baked Aucklandia lappa Decne., which have different therapeutic effect in clinical application. In this study, based on color measurement and fingerprint analysis, the method to assess the quality of these two processed products was established. In color measurement, the reference ranges of color parameters (L*, a*, and b*), standard color difference values, and mathematical prediction functions of these two processed products were obtain after the color was measured by a spectrophotometer. Meanwhile, high‐performance liquid chromatography fingerprints of these two processed products were established, where there were 12 peaks recognized as the common peaks in both processed products, in which two peaks were identified as costunolide and dehydrocostus lactone, and these two processed products were classified with chemometrics analysis subsequently. Furthermore, the correlation between color parameters and sample compositions was explored and the contents of costunolide and dehydrocostus lactone were determined simultaneously by high‐performance liquid chromatography. Consequently, an integral method including color measurement, high‐performance liquid chromatography fingerprint with chemometrics analysis, and quantitative determination was established.
Fructus Gardeniae, known as Zhi-zi in China, has been used as Chinese herbal medicine and functional health food for thousands of years. Fructus Gardeniae Grandiflorae, named as Shui-zhizi, is a counterfeit herb of Fructus Gardeniae. In order to discriminate these two varieties, based on ultra-high-performance liquid chromatography, an analysis method of fingerprints of Fructus Gardeniae and Fructus Gardeniae Grandiflorae was established. With hierarchical clustering analysis and principal component analysis, they were separated into two groups. Analyzed with partial least squares discriminant analysis, there were differences in chemical compositions between Fructus Gardeniae and Fructus Gardeniae Grandiflorae. Six compounds, crocin I, genipin-1-β-D-gentiobioside and four other unknown compositions were identified as differential marker compositions between them. Furthermore, seven active substances in them were determined simultaneously. Thus, an integral method of ultra-high-performance liquid chromatography fingerprint combined with chemometrics analysis and quantitative assessment was established. It could be utilized in characterization, quality evaluation of Fructus Gardeniae and could be applied for discriminating Fructus Gardeniae from Fructus Gardeniae Grandiflorae.
Introduction Zingiberis Rhizoma (ZR) has been used as a traditional Chinese herb and culinary food for thousands of years. Its two processed products, Zingiberis Rhizoma Praeparatum (ZRP) and carbonised ginger (CG), possess different therapeutic effects. Objectives To establish an objective and comprehensive method to differentiate ZRP from CG and to evaluate their qualities. Methodology Colour values of ZRP and CG were tested to establish the colour models by spectrophotometry. Moreover, high‐performance liquid chromatography (HPLC) was developed for fingerprint and quantitative analysis, and chemometric approaches were applied to discriminate between ZRP and CG. Finally, Spearman's correlation analysis was performed to investigate the relationship between the colour values and the peak areas of the common chemical compositions. Results Colour reference ranges of colour parameters and mathematical functions were built to distinguish ZRP from CG. In fingerprint analysis, 26 common peaks were detected in these two processed products, among which 6‐gingerol, 8‐gingerol, 6‐shogaol, 10‐gingerol, 8‐shogaol and 10‐shogaol were identified. Meanwhile, ZRP could be differentiated from CG by chemometrics analysis. In addition, the correlation between colour parameters and common peak areas was found and the contents of 6‐gingerol, 8‐gingerol, 6‐shogaol, 10‐gingerol, and 8‐shogaol were determined simultaneously. Conclusions An objective approach of colour measurement, HPLC fingerprint coupled with chemometrics analysis and quantitative assessment could be applied to discriminate ZRP from CG and evaluate the qualities of ZRP and CG rapidly.
Introduction: Magnoliae officinalis cortex (MOC), a traditional Chinese medicine, has been used in treating gastrointestinal diseases since ancient time. According to the Chinese Pharmacopoeia, it includes two kinds of decoction pieces, raw and ginger juice processed Magnoliae officinalis cortex (RMOC and GMOC).Objective: The aim of this paper was to study the differences between non-volatile and volatile components in RMOC and GMOC. Methods:The non-volatile components were detected by HPLC fingerprinting coupled with content determination (syringin, magnoflorine, honokiol and magnolol).Meanwhile, their odor information was obtained using a Heracles NEO ultra-fast gas phase electronic nose to conduct radar fingerprint analysis, principal component analysis and discriminant factor analysis, and the volatile components were analyzed qualitatively by the Kovats retention index and the AroChemBase database.Results: The HPLC fingerprints were established and 20 common peaks were found in all chromatograms with similarity values of more than 0.900. The content determination results showed that the contents of syringin and magnoflorine decreased, while the contents of honokiol and magnolol increased in GMOC. By the gas phase electronic nose, the two decoction pieces could be distinguished obviously and 16 possible compounds were identified. Among them, the relative contents of (À)-α-pinene and β-pinene increased, while β-phellandrene and (+)-limonene levels decreased. Conclusion:The results suggested that honokiol, magnolol, (À)-α-pinene and β-pinene might be the main substances which could enhance the harmonizing effect on the stomach. Moreover, this paper could lay a foundation for exploring the processing mechanism of MOC and provide a novel method for the research of other traditional Chinese medicine with strong aroma.
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