Comparative pharmacokinetic and bioavailability study of lobetyolin in rats after administration of lobetyolin and Codonopsis pilosula extract by ultra‐performance LC–tandem mass spectrometry

Abstract

Codonopsis pilosula (CP) is a traditional Chinese medicine used to invigorate spleen, replenish lung, nourish blood and engender fluid. A rapid, selective and sensitive ultra-performance LC-tandem mass spectrometry method was developed and validated to determine lobetyolin in rat plasma. The calibration curve showed good linearity over a concentration range of 0.46-1000 ng/mL for lobetyolin. The extraction recovery ranged from 72.5% to 89.1% with matrix effects of 81.6%-107.8%.The intra-and inter-batch precision and accuracy were 0.02-14.4% and −13.9% to −1.36%, respectively. The method was successfully applied for the bioavailability study of lobetyolin in rats after oral administration of pure lobetyolin and CP extract.Results showed that the elimination half-time (t 1/2 ) and the area under the concentration-time curve from zero to infinity of lobetyolin in CP extract were statistically different from those of the pure monomer (P < 0.05). However, the time to reach the maximum plasma concentration (T max ) and the maximum concentration (C max ) showed no significant differences between the two treatments. Furthermore, the bioavailability of lobetyolin in the experimental group was only 3.90%, significantly lower than that of the CP extract group (6.97%). The low bioavailability indicated that this component may be absorbed poorly or metabolized extensively in rats. Our results will provide useful information for further preclinical studies and formulation preparation of lobetyolin.

The Effect and Mechanism of New Processing Method of Codonopsis pilosula on Endocrine Physique Index in Rats

“…In addition, CP can reduce erythrocyte sclerotic index, increase blood fluidity, promote human microcirculation, and significantly inhibit the formation of thrombus in vitro [ 21 ]. In addition, CP may also reduce blood pressure by dilating blood vessels and increasing the output of the heart, brain, viscera, and other organs [ 22 ]. The pharmacological activity of TCM polysaccharides is obvious, among which CP polysaccharides can protect gastric tissue, increase pepsin activity and excretion, increase intestinal peristalsis, and promote digestion.…”

Untargeted Metabolomics Reveals Multiple Phytometabolites in the Agricultural Waste Materials and Medicinal Materials of Codonopsis pilosula

“…Referring to the method recorded in the China Pharmacopoeia Committee (2020) and previous study ( Dong et al, 2021 ), dried aerial parts of C. pilosula were used for lobetyolin extraction by the ultrasound-assisted method. Briefly, 1 g powder was transferred to a conical flask with 50 mL of methanol.…”

Utilization of functional agro-waste residues for oyster mushroom production: Nutritions and active ingredients in healthcare

“…Lobetyolin is a standard composition to evaluate the quality of Radix Codonopsis. According to the Chinese Pharmacopoeia (2020) and the previous studies ( Dong et al., 2021 ; Zeng et al., 2022b ), lobetyolin in oyster mushrooms from CSL and control group was extracted by the ultrasound-assisted method. In brief, 10 g of each dried samples was precisely weighed and introduced into a conical flask with 50 mL of methanol.…”