This study, conducted in Anchieta in the south of the state of Espírito Santo, aimed to investigate the presence of brucellosis in cattle slaughtered in a slaughterhouse inspected by the State Inspection Service (S.I.E) and the association between the lesions found in the cervical mucous membrane and the disease. Were collected 1081 blood samples in the period from february to August 2013, of these, 38 (3.52 %) specimens showed granulomatous lesions in the cervical mucous membrane type, cervical ligament and possibly the adjacent musculature. Of these, only 02 (5.26 %) samples were positive for brucellosis in rose Bengal test (AAT), however of the 1034 (95.65%) samples without injury 09 (0.86%) also showed positive in the AAT. To find out whether there was a association between the lesions found and the presence of brucellosis (P>0.05). These data do not explain the association of brucellosis with granulomatous lesions found along the cervical ligament in post-mortem inspection, which suggests the presence of an agent other than Brucella abortus
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