Herein we investigate the structure/function relationships of fucoidans from Ascophyllum nodosum to analyze their pro-angiogenic effect and cellular uptake in native and glycosaminoglycan-free (GAG-free) human endothelial cells (HUVECs). Fucoidans are marine sulfated polysaccharides, which act as glycosaminoglycans mimetics. We hypothesized that the size and sulfation rate of fucoidans influence their ability to induce pro-angiogenic processes independently of GAGs. We collected two fractions of fucoidans, Low and Medium Molecular Weight Fucoidan (LMWF and MMWF, respectively) by size exclusion chromatography and characterized their composition (sulfate, fucose and uronic acid) by colorimetric measurement and Raman and FT-IR spectroscopy. The high affinities of fractionated fucoidans to heparin binding proteins were confirmed by Surface Plasmon Resonance. We evidenced that LMWF has a higher pro-angiogenic (2D-angiogenesis on Matrigel) and pro-migratory (Boyden chamber) potential on HUVECs, compared to MMWF. Interestingly, in a GAG-free HUVECs model, LMWF kept a pro-angiogenic potential. Finally, to evaluate the association of LMWF-induced biological effects and its cellular uptake, we analyzed by confocal microscopy the GAGs involvement in the internalization of a fluorescent LMWF. The fluorescent LMWF was mainly internalized through HUVEC clathrin-dependent endocytosis in which GAGs were partially involved. In conclusion, a better characterization of the relationships between the fucoidan structure and its pro-angiogenic potential in GAG-free endothelial cells was required to identify an adapted fucoidan to enhance vascular repair in ischemia.

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Knowledge of the paleo-stress distribution is crucial to understand the fracture set up and orientations during the tectonic evolution of a basin, and thus the corresponding fluid flow patterns in a reservoir. This study aims to predict the main stress orientations and evolution during the growth of a fold by using the limit analysis method. Fourteen different steps have been integrated as 2D cross sections from an early stage to an evolved stage of a schematic and balanced propagation fold. The stress evolution was followed during the time and burial of syn tectonic layers localized in front of the thrust. Numerical simulations were used to predict the occurrence and orientation of deformation bands, i.e., compaction and shear bands, by following the kinematic of a fault-propagation fold. The case study of the Sant-Corneli-Boixols anticline was selected, located in the South Central Pyrenees in the Tremp basin, to constrain the dimension of the starting models (or prototypes) used in our numerical simulations. The predictions of the numerical simulations were compared to field observations of an early occurrence of both pure compaction- and shear-enhanced compaction bands in the syn-tectonic Aren formation located in front of the fold, which are subjected to early layer parallel shortening during the burial history. Stress magnitude and stress ratio variations define the type of deformation band produced. Our results show that the band occurrence depends on the yield envelope of the host material and that a small yield envelope is required for these shallow depths, which can only be explained by the heterogeneity of the host rock facies. In our case, the heterogeneity can be explained by a significant contribution of carbonate bioclasts in the calcarenite rock, which change the mechanical behavior of the whole rock.

Well dated, and precisely situated in the Salle Monique of the Cave of La Vache at Alliât (Ariège), this object, complete, from the R. Robert Collection, is an exceptionnal work of art. His decoration opens up a wide range for research and establishes the question of its interpretation.

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