Proteinases from hepatopancreas (HP) and gastric juice (GJ) from wild and cultured red octopus (Octopus maya) were characterized. Hepatopancreas assays revealed optimal activity at pH 4, 9-10 and 10 for wild and pH 3, 8, and 9, for cultured octopuses, for total proteinases, trypsin and chymotrypsin, respectively. In the gastric juice, maximum activity was recorded at pH 6, 8, and 7 for total proteinases, trypsin, and chymotrypsin, respectively for both wild and cultured octopus. A reduction on enzyme activity of 70 and 20% was observed in HP and GJ extracts, respectively when protease inhibitor Pepstatin A was used. That result suggests that the main proteases in the HP were aspartic acid proteinases type (possibly Cathepsin D) and some of them were present in the GJ. Dissociating discontinuous polyacrylamide gel electrophoresis showed activity bands between 20 and 28, 30 and 34, 35 and 45, 60 and 70 kDa, and a last one between 75 and 100 kDa. We concluded that extracellular digestion of O. maya takes place in an acid environment, around pH 6. In contrast, intracellular digestion in the HP is developed at pHs between 3 and 4, where cathepsin D could be the most important enzyme for O. maya.

In-season session training load relative to match load in professional ice hockey. J Strength Cond Res 36(2): 486-492, 2022-The aim of this study was to describe match load and intensity across player positions and match periods, the distribution of pregame training load and intensity over training days before a match, by player position, and the cumulative weekly training load over a season. Fifty professional ice hockey players, which at one time or another were part of the roster for the Laval Rocket during the 2017-18 season of the American Hockey League, participated in this study. External load was monitored in every training session and match over the season using portable inertial measurement units. Data are reported in absolute and relative values to a match. Defensemen have a lower intensity during matches than forwards (21.70 OIL•min 21 ) while load is similar across position. The first period shows a higher relative load (+5.28% relative on-ice load [OIL]) while the third has a lower relative intensity (22.91% OIL•min 21 ). Defensemen seem to train at a systematic higher relative intensity than wingers and centers (+8.34% relative OIL•min 21 ). Finally, the weekly training load remains relatively constant throughout the season (equivalent of 3.56 6 1.69 matches played per week). Our results support the need for player monitoring in ice hockey using an individualized approach.

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