HARDERIAN GLAND PORPHVRIN ENZYMES 775 teroides and S. aureus infected animals whose survival times were significantly prolonged. In vitro, however, 4-chlorotestosterone did not aweus, hastens healing of cutaneous nocardosis in rabbits, and nullifies the proinfective action of cortisone. inhibit development of the infecting strains. According to these data the particular antiinfective action of 4-chlorotestosterone is not attributed to a direct chemotherapeutic effect but rather to a stimulating action of the steroid on organic reactive systems; particularly those inhibited by cortisone and conditioning the organic response to allergic or infective stimuli.Summary. 4-chlorotestosterone is an anabolic steroid comparable to testosterone, but with less androgenic potential. It enhances the intensity of anaphylactic shock in guinea pigs, prolongs survival time of mice infected with Nocwdia asteroides, or Staphylococcus 1. Taubenhaus, M., Amromin, G. D., Endocri-2. ___. , J . Lab. Clin. Med., 1950, v36, 7. 3. Taubenhaus, M., Symposium uber die Beeinflussung der reaktiven Geschehens durch Hypophyse and Nebennieuenrinde, Zurich 1951. Benno Schlwabe Verlag, Basel, p54.It was observed that the Harderian glands of rats, hamsters, and most mice fluoresced red, while the Harderian glands of rabbits and guinea pigs did not ( 1-5). I t had been assumed, and was later found, that the red fluorescent Harderian glands conltained relatively greater quantities of porphyrin than those that did not fluoresce red. The enzymes for producing porphyrin must, however, be present in all Harderian glands because free protoporphyrin has been extracted from Harderian glands that were not red fluorescent. It was, therefore, of interest to determine the quantitative aspects of porphyrin producing enzymes in Harderian glands of various rodents. It was especially desirable to compare amounts of enzyme activity present in rat and hamster, where the glands are *This work was supported by grants from Anna Fuller Fund, Am. Cancer SOC., Md. Division, and S.I.H., P.H.S. (CY358O). t Submitted in partial fulfillment of requirements for degree of Doctor of Philosophy.usually red fluorescent, with the amount of enzyme in Harderian glands in guinea pigs and rabbits, where these glands are usually not red fluorescent. In some rodents, where Harderian glands are white fluorescent, the porphyrin is present in such small quantities that its red fluorescence is masked by presence of blue-green fluorescent maiterial. The bluegreen and red fluorescence spectra are complimentary and the glands appear to be white fluorescent. In the rabbit, the Harderian gland consists of a dark and a white fluorescent portion. The white fluomrescent portion contains by far the most porphyrin, but not nearly as much as the Harderian glands of rats and hamsters.The results of these experiments on quantitative aspects of porphyrin producing enzymes in Harderian glands of rodents indicate that while these enzymes were present in rabbit and guinea pig Harderian glands, the enzyme acti...