Application of antioxidants in the cosmetic industry demands control of the efficiency of ROS-scavenging within the cream matrix. Our goal was to construct a system for the simultaneous detection of superoxide and hydrogen peroxide and their possible scavengers. DMSO is a good solvent for many cosmetic products, and thus the system should work in mixed aqueous-organic media. The fluidic chip developed consists of an ROS-generation chamber, a mixing section and a compartment for the biosensor chip. This electrode chip had two sensors: one sensor for each species. Cytochrome c was used as the sensing protein. Both the superoxide and the hydrogen peroxide sensors demonstrated sufficient sensitivity in DMSO-buffer mixtures within the concentration range 0.4 nM-1.2 nM (superoxide) and 50 microM-1000 microM (hydrogen peroxide). The influence of the flow conditions on the generation of ROS was investigated and the optimal parameters for the antioxidant detection were evaluated. The efficiency of ROS-scavenging was tested with typical antioxidants of enzymatic and non-enzymatic origin, as well as complex cosmetic creams.
Bioanalytical THz sensing techniques have proven to be an interesting and viable tool for the label-free detection and analysis of biomolecules. However, a major challenge for THz bioanalytics is to perform investigations in the native aqueous environments of the analytes. This review recapitulates the status and future requirements for establishing THz biosensing as a complementary toolbox in the repertoire of standard bioanalytic methods. The potential use in medical research and clinical diagnosis is discussed. Under these considerations, this article presents a comprehensive categorization of biochemically relevant analytes that have been investigated by THz sensing techniques in aqueous media. The detectable concentration levels of ions, carbohydrates, (poly-)nucleotides, active agents, proteins and different biomacromolecules from THz experiments are compared to characteristic physiological concentrations and lower detection limits of state-of-the-art bioanalytical methods. Finally, recent experimental developments and achievements are discussed, which potentially pave the way for THz analysis of biomolecules under clinically relevant conditions.
Sczech, R.; Gómez Rivas, J.; Berrier, A.; Giannini, V.; Pirruccio, G.; Debus, C.; Schäfer-Eberwein, H.; Haring Bolivar, P. Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publicationCitation for published version (APA): Sczech, R., Gómez Rivas, J., Berrier, A., Giannini, V., Pirruccio, G., Debus, C., ... Haring Bolivar, P. (2012). Long-range guided THz radiation by thin layers of water. Optics Express, 20(25), 27781-27791. DOI: 10.1364/OE.20.027781 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract:We propose a novel method to guide THz radiation with low losses along thin layers of water. This approach is based on the coupling of evanescent surface fields at the opposite sides of the thin water layer surrounded by a dielectric material, which leads to a maximum field amplitude at the interfaces and a reduction of the energy density inside the water film. In spite of the strong absorption of water in this frequency range, calculations show that the field distribution can lead to propagation lengths of several centimeters. By means of attenuated total reflection measurements we demonstrate the coupling of incident THz radiation to the long-range surface guided modes across a layer of water with a thickness of 24 μm. This first demonstration paves the way for THz sensing in aqueous environments.
We demonstrate end-fire excited THz radiation coupled in thin layers of water propagating a distance of 4 cm. As the propagation lengths are critically dependent from the permittivity, we experimentally demonstrate this propagation length dependency by stepwise exchanging the material layer from deionized water to pure ethanol.
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