Horseweed (Conyza canadensis) is a cosmopolitan weed that commonly grows throughout North America. Horseweed that is not completely controlled by normal applications of glyphosate has been reported in western Tennessee. This research had three objectives: (1) to develop and validate an analytical procedure for the quantitative determination of shikimate, an important indicator of glyphosate activity in plants; (2) to confirm resistance to glyphosate in a horseweed population; and (3) to examine the accumulation of shikimate in both glyphosate-resistant and glyphosate-susceptible horseweed plants. The analytical procedure to determine shikimate used extraction with 1 M HCl for 24 h, followed by liquid chromatography using photodiode array detection, and shikimate recoveries were >or=82%. Glyphosate applications of both 0.84 kg ae/ha (the standard application rate) and 3.8 kg ae/ha to susceptible plants caused complete plant death. The same glyphosate applications to putative resistant populations caused less than 15% growth reduction as determined by visual evaluations, and fresh weights of these resistant plants 17 days after glyphosate treatment (DAT) were reduced an average of 45% in one population and were not affected in a different population. This direct comparison conclusively confirms that horseweed plants collected in western Tennessee in 2002 are resistant to 4 times the normal application dosage of glyphosate. The glyphosate-resistant horseweed biotypes still exhibited some herbicidal effects from the glyphosate, such as yellowing in the most actively growing, apical shoot meristems. The yellowing in the shoot apexes was transitory, and the plants recovered from this damage. Shikimate concentrations in all untreated horseweed plants were less than 100 microg/g, which was significantly less than that in all plants which had been treated with 0.84 kg ae/ha of glyphosate. Unexpectedly, shikimate accumulated (>1000 microg/g) in both resistant populations and the susceptible population. However, there were differences in shikimate accumulation patterns between resistant and susceptible horseweed biotypes. Shikimate concentrations in resistant populations declined about 40% from 2 to 4 DAT, while shikimate concentrations in the susceptible horseweed plants increased about 35% from 2 to 4 DAT. The confirmed resistance of a widespread weed implies that alternative control strategies for glyphosate-resistant horseweed will be needed in those no-tillage production systems where it commonly occurs.
Survival of horseweed in several glyphosate-tolerant cotton and soybean fields treated with glyphosate at recommended rates preplant and postemergence was observed in Mississippi and Tennessee in 2001 and 2002. Plants originating from seed collected from fields where horseweed escapes occurred in 2002 were grown in the greenhouse to the 5-leaf, 13- to 15-leaf, and 25- to 30-leaf growth stages and treated with the isopropylamine salt of glyphosate at 0, 0.025, 0.05, 0.1, 0.21, 0.42, 0.84, 1.68, 3.36, 6.72, and 13.44 kg ae/ha to determine if resistance to glyphosate existed in any biotype. All biotypes exhibited an 8- to 12-fold level of resistance to glyphosate when compared with a susceptible biotype. One resistant biotype from Mississippi was two- to fourfold more resistant than other resistant biotypes. Growth stage had little effect on level of glyphosate resistance. The glyphosate rate required to reduce biomass of glyphosate-resistant horseweed by 50% (GR50) increased from 0.14 to 2.2 kg/ha as plant size increased from the 5-leaf to 25- to 30-leaf growth stage. The GR50rate for the susceptible biotype increased from 0.02 to 0.2 kg/ha glyphosate. These results demonstrate that the difficult-to-control biotypes were resistant to glyphosate, that resistant biotypes could survive glyphosate rates of up to 6.72 kg/ha, and that plant size affected both resistant and susceptible biotypes in a similar manner.
The relationship between corn (Zea mays L.) yield and plant height has been poorly documented in major corn production systems. This study was conducted to assess the relationship of corn yield with plant height under four major corn production systems at Milan, TN from 2008 through 2010. Six N treatments at rates of 0, 62, 123, 185, 247, and 308 kg N ha−1 with four replications were evaluated in a randomized complete block design in the following corn production systems: nonirrigated corn after corn, nonirrigated corn after soybean [Glycine max (L.) Merr.], nonirrigated corn after cotton [Gossypium hirsutum (L.)], and irrigated corn after soybean. The regression of corn yield with plant height was significant and positive at 6‐leaf growth stage (V6), 10‐leaf growth stage (V10), and 12‐leaf growth stage (V12), and mostly became stronger as plant growth progressed from V6 to V10 and to V12 under an exponential model in the four corn production systems for all 3 yr. In general, corn yield was strongly related with plant height measurements made at V10 and V12. Factors affecting the responses of plant height measured at V6, V10, and V12 or/and yield to the N treatments may have contributed to the variations of determination coeffient (R 2) values across years. In conclusion, corn yield may be predicted with plant height measurements collected during V10 to V12. This prediction provides a physiological basis for the utilization of high resolution plant height measurements to guide variable‐rate N applications within the field on corn at around V10 and to more accurately estimate yield for earlier grain marketing purposes.
Factors affecting horseweed emergence are important for management of this weed species, particularly because of the presence of herbicide-resistant biotypes. Horseweed emergence was highly variable and not strongly correlated to soil temperature (r 2 = 0.21), air temperature (r 2 = 0.45) or rainfall (r 2 = 0.32). Horseweed emerged mainly during April and September in Tennessee when average daytime temperatures fluctuate between 10 and 15.5 C. However, some horseweed plants emerged during almost any month when temperatures ranged from 10 to 25 C and adequate moisture was available at the soil surface. Horseweed densities ranged from a low of 30 to 50 plants m−2 to a high of > 1,500 plants−2 at one location. These extremely high densities illustrate the ability of horseweed to be an effective ruderal plant that can produce stands that approach monoculture densities if not controlled. The amount of crop residue remaining after harvest from the previous field season was in the order of corn > cotton > soybean > fallow. Residue from a previous corn crop reduced horseweed emergence compared with soybean and cotton residues in a no-tillage situation. Decreased horseweed density due to crop residue presence indicates that a systems approach may help reduce horseweed populations.
Sulfentrazone dissipation in soil was examined in field experiments in 1995, 1996, and 1997 at Knoxville, TN, on a Sequatchie loam soil. Sulfentrazone 50% disappearance time (DT50) varied from 24 to 113 d. Cotton injury was observed the year following sulfentrazone application when half-lives were ≥85 d. Sulfentrazone degradation under controlled laboratory conditions was slower in autoclaved soil than in nonautoclaved surface soil and subsurface soil, with DT50 of 198, 93, and 102 d, respectively. The difference due to autoclaving the soil implied that sulfentrazone degradation was influenced by both microbial and chemical mechanisms.
Horseweed (Conyza canadensis (L.) Cronq.) seed was collected in Illinois, Indiana, Kentucky, Mississippi, Missouri, and Ohio to determine susceptibility of different horseweed biotypes to glyphosate. Horseweed resistant to glyphosate was found in Mississippi, Ohio, and western Tennessee. In a separate experiment examining Tennessee biotypes, a dose response curve demonstrated that four times as much glyphosate was needed to achieve a 50% fresh weight reduction (GR(50)) in resistant biotypes when compared to a susceptible biotype. Resistant biotypes from Tennessee displayed a GR(50) of 1.6 kg/ha as compared to a GR(50) of 0.4 kg/ha in a susceptible horseweed population. Although growth was reduced, the resistant plants did not completely die and could potentially produce seed. Variation in glyphosate resistance was found among the populations tested.
Field studies were conducted to compare venturi-type nozzles to a fan nozzle with respect to the efficacy of postemergence herbicides applied to common cocklebur and broadleaf signalgrass. Spray solutions of glufosinate, glyphosate, and paraquat were applied through all combinations of three nozzles and two application volumes. Venturi nozzles were a Delavan Raindrop Ultra (RU) and a Spraying Systems AI Teejet (AI). A Spraying Systems XR Teejet (XR) fan nozzle was included as a standard. Previous work indicated droplet size spectra differed among these nozzles. There was a difference in common cocklebur control among nozzles (AI = XR > RU), although control was at least 90% for all nozzles. Herbicide choice had a greater effect on broadleaf signalgrass control than nozzle type. Broadleaf signalgrass control differed among herbicides (glufosinate = paraquat > glyphosate) and among nozzles (AI = XR > RU). Herbicide performance varied between nozzles (AI > RU), but the AI nozzle was as effective as the XR fan nozzle.
Broadleaf signalgrass is sensitive to nicosulfuron and resistant to primisulfuron, but corn is resistant to both. Research was conducted to determine the effect of varying light level and air temperature on absorption, translocation, and metabolism of nicosulfuron and primisulfuron in broadleaf signalgrass and corn. Corn absorbed between 60 and 85% of the applied nicosulfuron and primisulfuron within 72 h after treatment (HAT), depending on environmental treatment. Absorption, translocation, and metabolism all tended to be more rapid at higher temperature and light intensity. Nicosulfuron and primisulfuron translocation out of the treated leaf was < 4.5% of herbicide absorbed through 72 HAT. Corn rapidly metabolized both herbicides in both environments. However, primisulfuron was metabolized more rapidly (high = 99%, low = 92%) than nicosulfuron (high = 95%, low = 78%). Broadleaf signalgrass absorbed 20% more nicosulfuron than primisulfuron through 72 HAT. Nicosulfuron translocation out of the treated leaf in broadleaf signalgrass was ≤ 15% absorbed through 72 HAT, while primisulfuron translocation was ≤ 4% during the same time period. Primisulfuron metabolism was more rapid than nicosulfuron in broadleaf signalgrass. During the first 4 HAT, broadleaf signalgrass metabolized > 20 times more primisulfuron than nicosulfuron. By 72 HAT, broadleaf signalgrass under conditions of high light and temperature had metabolized nearly 90% of the primisulfuron absorbed but ≤ 7% of the nicosulfuron absorbed was metabolized during the same time. These results suggest that differential activity of nicosulfuron and primisulfuron on broadleaf signalgrass may be based on differential rates of metabolism to nonphytotoxic compounds; uptake and translocation differences agree with the differential broadleaf signalgrass activity. Additionally, environment has the potential to affect rates of sulfonylurea absorption, translocation, and metabolism.
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