The Earth's Critical Zone (CZ) is a thin living layer connecting atmosphere and geosphere, including aquifers. Humans live in the CZ and benefit from the vital supporting services it provides. However, the CZ is increasingly impacted by human activities including land and resource use, pollution, and climate change. Recent interest in uniting the many disciplines studying this complex domain has initiated an international network of research infrastructure platforms that allow access to the CZ in a range of geologic settings. In this paper a new such infrastructure platform associated with the Collaborative Research Center AquaDiva is described, that uniquely seeks to combine CZ research with detailed investigation of the functional biodiversity of the subsurface. Overall, AquaDiva aims to test hypotheses about how water connects surface conditions set by land cover and land management to the biota and biogeochemical functions in the subsurface. With long-term and continuous observations, hypotheses about how seasonal variations and extreme events at the surface impact subsurface processes, community structure and function are tested. AquaDiva has established the Hainich Critical Zone Exploratory (CZE) in central Germany in an alkaline geological setting of German Triassic Muschelkalk formations. The Hainich CZE includes specialized monitoring wells to access the vadose zone and two main groundwater complexes in limestone and marlstone parent materials along a ∼6 km transect spanning forest, pasture, and agricultural land uses. Initial results demonstrate fundamental differences in the biota and biogeochemistry of the two aquifer complexes that trace back to the land uses in their respective recharge areas. They also show the importance of antecedent conditions on the impact of precipitation events on responses in terms of groundwater dynamics, chemistry and ecology. Thus, we find signals of surface land use and events can be detected in the subsurface CZ. Future research will expand to a second CZE in contrasting siliciclastic parent rock, to evaluate the relative importance of parent material lithology vs. surface conditions for the emergent characteristics of the subsurface CZ Küsel et al.Tracing Surface Signals into Subsurface and biodiversity. The Hainich CZE is open to researchers who bring new questions that the research platform can help answer.
Despite the widely observed predominance of Cand . Patescibacteria in subsurface communities, their input source and ecophysiology are poorly understood. Here we study mechanisms of the formation of a groundwater microbiome and the subsequent differentiation of Cand . Patescibacteria. In the Hainich Critical Zone Exploratory, Germany, we trace the input of microorganisms from forested soils of preferential recharge areas through fractured aquifers along a 5.4 km hillslope well transect. Cand . Patescibacteria were preferentially mobilized from soils and constituted 66% of species-level OTUs shared between seepage and shallow groundwater. These OTUs, mostly related to Cand . Kaiserbacteraceae, Cand . Nomurabacteraceae, and unclassified UBA9983 at the family level, represented a relative abundance of 71.4% of the Cand . Patescibacteria community at the shallowest groundwater well, and still 44.4% at the end of the transect. Several Cand . Patescibacteria subclass-level groups exhibited preferences for different conditions in the two aquifer assemblages investigated: Cand . Kaiserbacteraceae surprisingly showed positive correlations with oxygen concentrations, while Cand . Nomurabacteraceae were negatively correlated. Co-occurrence network analysis revealed a central role of Cand . Patescibacteria in the groundwater microbial communities and pointed to potential associations with specific organisms, including abundant autotrophic taxa involved in nitrogen, sulfur and iron cycling. Strong associations among Cand . Patescibacteria themselves further suggested that for many groups within this phylum, distribution was mainly driven by conditions commonly supporting a fermentative life style without direct dependence on specific hosts. We propose that import from soil, and community differentiation driven by hydrochemical conditions, including the availability of organic resources and potential hosts, determine the success of Cand . Patescibacteria in groundwater environments.
Abstract. The quality of near-surface groundwater reservoirs is controlled, but also threatened, by manifold surfacesubsurface interactions. Vulnerability studies typically evaluate the variable interplay of surface factors (land management, infiltration patterns) and subsurface factors (hydrostratigraphy, flow properties) in a thorough way, but disregard the resulting groundwater quality. Conversely, hydrogeochemical case studies that address the chemical evolution of groundwater often lack a comprehensive analysis of the structural buildup. In this study, we aim to reconstruct the actual spatial groundwater quality pattern from a synoptic analysis of the hydrostratigraphy, lithostratigraphy, pedology and land use in the Hainich Critical Zone Exploratory (Hainich CZE). This CZE represents a widely distributed yet scarcely described setting of thin-bedded mixed carbonatesiliciclastic strata in hillslope terrains. At the eastern Hainich low-mountain hillslope, bedrock is mainly formed by alternated marine sedimentary rocks of the Upper Muschelkalk (Middle Triassic) that partly host productive groundwater resources. Spatial patterns of the groundwater quality of a 5.4 km long well transect are derived by principal component analysis and hierarchical cluster analysis. Aquifer stratigraphy and geostructural links were deduced from lithological drill core analysis, mineralogical analysis, geophysical borehole logs and mapping data. Maps of preferential recharge zones and recharge potential were deduced from digital (soil) mapping, soil survey data and field measurements of soil hydraulic conductivities (K s ). By attributing spatially variable surface and subsurface conditions, we were able to reconstruct groundwater quality clusters that reflect the type of land management in their preferential recharge areas, aquifer hydraulic conditions and cross-formational exchange via caprock sinkholes or ascending flow. Generally, the aquifer configuration (spatial arrangement of strata, valley incision/outcrops) and related geostructural links (enhanced recharge areas, karst phenomena) control the role of surface factors (input quality and locations) vs. subsurface factors (water-rock interaction, cross-formational flow) for groundwater quality in the multi-layered aquifer system. Our investigation reveals general properties of alternating sequences in hillslope terrains that are prone to forming multilayered aquifer systems. This synoptic analysis is fundamental and indispensable for a mechanistic understanding of ecological functioning, sustainable resource management and protection.
Microorganisms in groundwater play an important role in aquifer biogeochemical cycles and water quality. However, the mechanisms linking the functional diversity of microbial populations and the groundwater physicochemistry are still not well understood due to the complexity of interactions between surface and subsurface. Here, we used phospholipid fatty acids (PLFAs) relative abundances to link specific biochemical markers within the microbial communities to the spatio-temporal changes of the groundwater physicochemistry. PLFAs were isolated from groundwater of two physicochemically distinct aquifer assemblages in central Germany (Thuringia). The functional diversities of the microbial communities were mainly correlated with groundwater chemistry, including dissolved O2, Fet and NH4+ concentrations. Abundances of PLFAs derived from eukaryotes and potential nitrite oxidizing bacteria (11MeC16:0 as biomarker for Nitrospira moscoviensis) were high at sites with elevated O2 concentration where groundwater recharge supplies both bioavailable organic substrates and NH4+ needed to sustain heterotrophic growth and nitrification processes. In anoxic groundwaters more rich in Fet, PLFAs abundant in sulphate reducing bacteria (SRB), iron-reducing bacteria and fungi increased with Fet and HCO3− concentrations suggesting the occurrence of active iron-reduction and the possible role of fungi in meditating iron solubilisation and transport in those aquifer domains. In NH4+ richer anoxic groundwaters, anammox bacteria and SRB- derived PLFAs increased with NH4+ concentration further evidencing the dependence of the anammox process on ammonium concentration and potential links between SRB and anammox bacteria. Additional support of the PLFA-based bacterial communities was found in DNA and RNA-based Illumina MiSeq amplicon sequencing of bacterial 16S rRNA genes, which evidenced high predominance of nitrite-oxidizing bacteria Nitrospira e.g. Nitrospira moscoviensis in oxic zones of the aquifers and of anammox bacteria in NH4+ richer anoxic groundwater. Higher relative abundances of sequence reads in the RNA-based data sets affiliated with iron-reducing bacteria in Fet richer groundwater supported the occurrence of active dissimilatory iron-reduction. The functional diversity of the microbial communities in these biogeochemically distinct groundwater assemblages can be largely attributed to the redox conditions linked to changes in bioavailable substrates and input of substrates with the seepage. Our results demonstrate the power of complementary information derived from PLFA-based and sequencing-based approaches
Silicone polymers (polydimethylsiloxanes, or PDMS) are used in numerous personal care and household products, eventually enter wastewater treatment plants, and are later applied to the land as a component of sludge. The fate of silicones in soil is largely unknown, but this study shows that in a moist (0.2 MPa = 12% moisture) Londo sandy clay loam, 200 centi‐stoke (cs)14C‐labeled PDMS degraded slowly over six months to yield about 3% of applied 14C as low‐molecular‐weight, water‐soluble products. When the soil was allowed to dry in one week from 12 to 3% moisture, the degradation rate was much more rapid, and after several days at 3 % moisture about half of the applied 14C was water desorbable. HPLC‐GPC of tetrahydrofuran (THF) soil extracts showed that PDMS had been degraded to low‐molecular‐weight molecules of the general formula HO‐[Si(CH3)2O]n‐H. The range of moistures in this experiment was measured in a field of Londo sandy clay loam during the summer of 1992, indicating that PDMS should be unstable in the soil environment. Further work on the identification and biological degradation of these small products is ongoing.
Soil water content is a key variable for biogeochemical and atmospheric coupled processes. Its small-scale heterogeneity impacts the partitioning of precipitation (e.g., deep percolation or transpiration) by triggering threshold processes and connecting flow paths. Forest hydrologists frequently hypothesized that throughfall and stemflow patterns induce soil water content heterogeneity, yet experimental validation is limited. Here, we pursued a pattern-oriented approach to explore the relationship between net precipitation and soil water content. Both were measured in independent high-resolution stratified random designs on a 1-ha temperate mixed beech forest plot in Germany. We recorded throughfall (350 locations) and stemflow (65 trees) for 16 precipitation events in 2015. Soil water content was measured continuously in topsoil and subsoil (210 profiles). Soil wetting was only weakly related to net precipitation patterns. The precipitation-induced pattern quickly dissipates and returns to a basic pattern, which is temporally stable. Instead, soil hydraulic properties (by the proxy of field capacity) were significantly correlated with this stable soil water content pattern, indicating that soil structure more than net precipitation drives soil water content heterogeneity. Also, both field capacity and soil water content were lower in the immediate vicinity of tree stems compared to further away at all times, including winter, despite stemflow occurrence. Thus, soil structure varies systematically according to vegetation in our site. We conclude that enhanced macroporosity increases gravity-driven flow in stem proximal areas. Therefore, although soil water content patterns are little affected by net precipitation, the resulting soil water fluxes may strongly be affected. Specifically, this may further enhance the channelling of stemflow to greater depth and beyond the rooting zone
The binding of a weakly acidic herbicide (N‐2, 6‐difluorophenyl‐5‐methyl‐1,2,4‐triazolo(1,5a)pyrimidine‐2‐sulfonamide) to soil is presented as an ionization of the weak acid in solution, and the partitioning of both the anionic and neutral forms of the compound into the soil organic matter. This theoretical description is combined with experimentally determined bulk adsorption constants to give the organic C adsorption constants (Koc) for neutral (650 ± 40 L kg–1) and anionic (12 ± 1 L kg–1) forms of the molecule. Additional experiments with four soils adjusted to different pH levels gave an average Koc of 750 L kg±1 for the neutral form. Describing the soil sorption as a combination of anionic and neutral forms provided an adequate description of the measured sorption.
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