Water was withheld from wheat seedlings grown at two light levels, so that the soil water content was reduced by 50% and the leaf water potential of treated plants was about 0.5 MPa below that of controls. Growth was initially reduced but recovered after about 4 days of the water deficit treatment. Osmotic adjustment (calculated as the difference between the osmotic potential at 100% relative water content of the treated and controls plants) occurred in both the elongating and expanded zones of leaves. In plants grown at a very high irradiance, an increase in sugars accounted for 70-100% of the osmotic adjustment of 0.12-0.34 MPa in the elongating and expanded zones of leaves sampled both at dawn and at midday. The increased amounts of sugars were approximately equivalent to the decreased consumption of carbohydrate during the initial reduction in growth.
Glucose was the main component of the increase in sugars in the elongating regions, while sucrose was more important in the expanded laminae. During the morning, sugars increased to similar extents in controls and treated plants.
In plants grown at a moderate irradiance, sugars did not account for the osmotic adjustment of 0.10-0.21 MPa in the expanded laminae at dawn or at midday. At dawn, sugars were much lower in treated than in control plants but, during the morning, sugars accumulated more in the treated plants and sugar levels of the two groups at midday were similar. In contrast, sugar levels in the youngest elongating leaf of the treated plants were the same as in the controls at dawn, and were double those in the controls at midday.
After about 20 days, hypocotyl cuttings from 20-day-old loblolly pine (Pinus taeda L.) seedlings rooted easily in the presence of the auxin indole-3-butyric acid (IBA), with roots forming directly from xylem parenchyma. In contrast, woody cuttings from 1-2-year-old hedged seedlings formed roots indirectly from callus tissue in 60-90 days, but IBA had little effect on rooting. Variation in rooting among hypocotyls from both half- and full-sib families was highly significant in response to IBA, and rooting did not occur within 20 days unless IBA was applied. Hypocotyls from poor rooting families tended to produce fewer roots per cutting than hypocotyls from good rooting families. Rooting by woody cuttings and hypocotyl cuttings from the same nine full-sib families was weakly correlated, raising the possibility that at least some common genetically controlled processes were affecting rooting by both types of cutting. The phytotropin N-1-naphthylphthalamic acid (NPA), supplied at 1 micro M with 10 micro M IBA, significantly inhibited rooting by hypocotyl cuttings from both good and poor rooting families, but there was no significant family x treatment interaction. Family variation in rooting ability may be a function of the frequency of occurrence of auxin-responsive cells in the hypocotyls.
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