In the context of emergency warnings, auditory icons, which convey information about system events by analogy with everyday events, have the potential to be understood more quickly and easily than abstract sounds. To test this proposal, an experiment was carried out to evaluate the use of auditory icons for an in-vehicle collision avoidance application. Two icons, the sounds of a car horn and of skidding tyres, were compared with two conventional warnings, a simple tone and a voice saying 'ahead'. Participants sat in an experimental vehicle with a road scene projected ahead, and they were required to brake in response to on-screen collision situations and their accompanying warning sounds. The auditory icons produced significantly faster reaction times than the conventional warnings, but suffered from more inappropriate responses, where drivers reacted with a brake press to a non-collision situation. The findings are explained relative to the perceived urgency and inherent meaning of each sound. It is argued that optimal warnings could be achieved by adjusting certain sound attributes of auditory icons, as part of a structured, user-centred design and evaluation procedure.

A study was conducted to assess the relative impact of partial sleep deprivation (restriction to 4 h sleep before testing) and full sleep deprivation (no sleep on the night before testing) on 2 h of simulated driving, compared with an alcohol treatment (mean blood alcohol content = 0.07%). Data were collected from the 64 male participants on the primary driving task, psychophysiology (0.1 Hz heart rate variability), and subjective self-assessment. The results revealed that the full sleep deprivation and alcohol group exhibited a safety-critical decline in lane-keeping performance. The partial sleep deprivation group exhibited only noncritical alterations in primary task performance. Both sleep-deprived groups were characterized by subjective discomfort and an awareness of reduced performance capability. These subjective symptoms were not perceived by the alcohol group. The findings are discussed with reference to the development of systems for the online diagnosis of driver fatigue. Potential applications of this research include the formulation of performance criteria to be encompassed within a driver impairment monitoring system.

Applications of speech recognition are now widespread, but user-centred evaluation methods are necessary to ensure their success. Objective evaluation techniques are fairly well established, but previous subjective techniques have been unstructured and unproven. This paper reports on the first stage in the development of a questionnaire measure for the Subjective Assessment of Speech System Interfaces (SASSI). The aim of the research programme is to produce a valid, reliable and sensitive measure of users' subjective experiences with speech recognition systems. Such a technique could make an important contribution to theory and practice in the design and evaluation of speech recognition systems according to best human factors practice. A prototype questionnaire was designed, based on established measures for evaluating the usability of other kinds of user interface, and on a review of the research literature into speech system design. This consisted of 50 statements with which respondents rated their level of agreement. The questionnaire was given to users of four different speech applications, and Exploratory Factor Analysis of 214 completed questionnaires was conducted. This suggested the presence of six main factors in users' perceptions of speech systems: System Response Accuracy, Likeability, Cognitive Demand, Annoyance, Habitability and Speed. The six factors have face validity, and a reasonable level of statistical reliability. The findings form a useful theoretical and practical basis for the subjective evaluation of any speech recognition interface. However, further work is recommended, to establish the validity and sensitivity of the approach, before a final tool can be produced which warrants general use.

One in 4 prescription medication users took an NVDS in the prior 12 months, yet the majority did not share this with a conventional medical professional.

Basally located tight junctions between Sertoli cells in the postpubertal testis are the largest and most complex junctional complexes known. They form at puberty and are thought to be the major structural component of the "blood-testis" barrier. We have now examined the development of these structures in the immature mouse testis in conjunction with immunolocalization of the tight-junction-associated protein ZO-1 (zonula occludens 1). In testes from 5-day-old mice, tight junctional complexes are absent and ZO-1 is distributed generally over the apicolateral, but not basal, Sertoli cell membrane. As cytoskeletal and reticular elements characteristic of the mature junction are recruited to the developing junctions, between 7 and 14 days, ZO-1 becomes progressively restricted to tight junctional regions. Immunogold labeling of ZO-1 on Sertoli cell plasma membrane preparations revealed specific localization to the cytoplasmic surface of tight junctional regions. In the mature animal, ZO-1 is similarly associated with tight junctional complexes in the basal aspects of the epithelium. In addition, it is also localized to Sertoli cell ectoplasmic specializations adjacent to early elongating, but not late, spermatids just prior to sperm release. Although these structures are not tight junctions, they do have a similar cytoskeletal arrangement, suggesting that ZO-1 interacts with the submembrane cytoskeleton. These results show that, in the immature mouse testis, ZO-1 is present on the Sertoli cell plasma membrane in the absence of recognizable tight junctions. In the presence of tight junctions, however, ZO-1 is found only at the sites of junctional specializations associated with tight junctions and with elongating spermatids.

Background:Beneficial correlations are suggested between food preparation and home food preparation of healthy choices. Therefore, there is an emergence of culinary medicine (CM) programs directed at both patients and medical professionals which deliver education emphasizing skills such as shopping, food storage, and meal preparation.Objective:The goal of this article is to provide a description of emerging CM programs and to imagine how this field can mature.Methods:During April 2015, 10 CM programs were identified by surveying CM and lifestyle medicine leaders. Program directors completed a narrative describing their program's structure, curricula, educational design, modes of delivery, funding, and cost. Interviews were conducted in an effort to optimize data collection.Results:All 10 culinary programs deliver medical education curricula educating 2654 health professionals per year. Educational goals vary within the domains of (1) provider's self-behavior, (2) nutritional knowledge and (3) prescribing nutrition. Six programs deliver patients' curricula, educating 4225 individuals per year. These programs' content varies and focuses on either specific diets or various culinary behaviors. All the programs' directors are health professionals who are also either credentialed chefs or have a strong culinary background. Nine of these programs offer culinary training in either a hands-on or visual demonstration within a teaching kitchen setting, while one delivers remote culinary tele-education. Seven programs track outcomes using various questionnaires and biometric data.Conclusions:There is currently no consensus about learning objectives, curricular domains, staffing, and facility requirements associated with CM, and there has been little research to explore its impact. A shared strategy is needed to collectively overcome these challenges.

Sodium-potassium ATPase (Na+K(+)-ATPase) is a ubiquitous plasma membrane enzyme which uses the hydrolysis of ATP to regulate cellular Na+ and K+ levels and fluid volume. This ion pumping action is also thought to be involved in fluid movement across certain epithelia. There are several different genes for this enzyme, some of which are tissue specific. Using an antibody specific for the catalytic subunit of canine kidney Na+K(+)-ATPase, we have localized immunoreactivity in the seminiferous and epididymal epithelium of rats of various ages. There was no specific staining of 10-day-old rat testis. Faint staining was detected at 13 days and appeared to be associated with the borders of Sertoli cells. At 16 days prominent apical and lateral staining but no basal staining of Sertoli cell membranes was observed. This type of distribution continued until spermatids were present in the epithelium. In the adult rat testis, specific staining was detected in Sertoli cell crypts associated with elongating spermatids, and on the apical and lateral Sertoli cell membrane. In some instances immunoreactivity was concentrated at presumed sites of junctional specializations. In the excurrent ducts of immature and mature rats, Na+K(+)-ATPase staining was heavy in the efferent ducts and somewhat lighter in the epididymis. In all regions, the staining was basolateral although there were variations in intensity among the different parts of the epididymis. These results show 1) that rat testis and epididymal Na+K(+)-ATPase share some immunological determinants with the canine enzyme; 2) that the epididymal enzyme is located in the conventional basolateral position; and 3) that the distribution of Sertoli cell Na+K(+)-ATPase is probably apical and lateral rather than basal.

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